Sequences from confirmation amplicon


Confirmation sequences for line 328C08 and hit At2g32320 are shown.
Line ID 328C08
Locus At2g32320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13731600
Primers used for PCR TA23 - 8474, show primer details
   
Sequence Name 13-K030139-022-328-C08C-8409
GenBank Accession LN491910 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K030139-022-328-C08C-8409
AGCCATTTACAATTGAAAAAGGTTAATTTAAAATAATGGGAAAAAAACAAAAAATAGTGA
AAGAAAAAAGTAAACCATTCGGTTCGATTGTTTTGTACTTTAGCTTCGGTTTATCAAAAT
AAGAGCTGAATCTCATTCGGTTTTCGGTTTGGAATTTTTTTTTGGTAGCAATGAATACCT
GTCTTAGGAGAAGCTAAGAATGTGAGGATGTTGTTGCCAGAAACATTGGTCGATAATGTT
TGAATAGTCTACGCCCACTTCTGCTTCCACTTGCTTTCCTGAAACTTCTCCATTCTCCTC
TGTAACACCTTCCTGCGTCTAAATGTAAAAACTATTTCAGACCACTTATTTCAGACCACT
TGTTTAAAACTATTTCAGACCACTTGTAAAAACAAGAATGCCTTAAAAGGTTTCTCTTGA
AAGGACCCTTCTTTTCTTCACAATAATAAGAATCTGCTATGAAACACTAGAAGGATTTTG
GAGTATGATAATAAAAGTAGCACCTTTAGGCGGAAAACGGAAGAACCCATGCGGAAGATT
ACAGGTAATGAATTGTACTCAATTCCAAATTGCTG
   
Sequence Name 13-K030139-022-328-C08C-TA23
GenBank Accession LN491911 [GenBank]
Sequencing primer gene specific primer, TA23: 5'-AGTTACTTAGCCAGCAATTTGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K030139-022-328-C08C-TA23
TATCTTCCGCACTGGGTTCTTCCGCTTTTCCGCCTAAAGGTGCTACTTTTATTATCATAC
TCCAAAATCCTTCTAGTGTTTCATAGCAGATTCTTATTATTGTGAAGAAAAGAAGGGTCC
TTTCAAGAGAAACCTTTTAAGGCATTCTTGTTTTTACAAGTGGTCTGAAATAGTTTTAAA
CAAGTGGTCTGAAATAAGTGGTCTGAAATAGTTTTTACATTTAGACGCAGGAAGGTGTTA
CAGAGGAGAATGGAGAAGTTTCAGGAAAGCAAGTGGAAGCAGAAGTGGGCGTAGACTATT
CAAACATTATCGACCAATGTTTCTGGCAACAACATCCTCACATTCTTAGCTTCTCCTAAG
ACAGGTATTCATTGCTACCAAAAAAAAATTCCAAACCGAAAACCGAATGAGATTCAGCTC
TTATTTTGATAAACCGAAGCTAAAGTACAAAACAATCGAACCGAATGGTTTACTTTTTTC
TTTCACTATTTTTTGTTTTTTTCCATTATTTTAAATTAACCTTTTTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTAT
AAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAA
GCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11