Sequences from confirmation amplicon


Confirmation sequences for line 333E05 and hit At2g25540 are shown.
Line ID 333E05
Locus At2g25540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10869767
Primers used for PCR WS04 - 8474, show primer details
   
Sequence Name 26-K019822-022-333-E05C-8409
GenBank Accession LN497717 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K019822-022-333-E05C-8409
TGATCCATGTAGATTTCCCGGACATGAAGCCATTTACATGAAGCTCATGAACCTAGAGCT
CCTGAGTTCTATTTTTCCCAGAAGATAGATTACTTGAAGGACAAGATCCAACCTTCTTTT
GTCAAAGAGAGACGGGCAATGAAGGTCACTTGGACTACTATAATTGCATAAATAGCTTCT
GCTTGTTTTATCCATTAGACATATATACGAGACTAAAGAGTGAATGTCACATTTTCTTAT
GTCCCACGTTGGCAGAGAGAGTATGAGGAGTTTAAAGTGAGGATCAATATCCTCGTCGCT
AAAGCACAGAAGATTCCTGAAGATGGTTGGACAATGGAGGATGGTACGTCCTGGCCTGGT
AACAACCCTAGAGACCATCCTGGGATGATTCAGGTACACTAGTATAATCACTTCCTTTTA
TAGTAATTCTCCTAGATGGGCAAATATTTATCTATAATGTTTGTTGATTCTAATTATTTT
GTTACAATTCAGGTTTTCTTAGGCCATAGTGGAGGTCTGGATACCGATGGAAATGAGCTA
CCTAGGCTCATCTATGTGTCCCGTGAAAAGCGTCCTGGATTTCAGCACCACAAGAAGGCT
GGAGCTATGAATGCCTTGGTTCGTTTTATCTTTCGTAAATCTGTTGTGCTCTCTTTTGTT
CAATGGCTTGGAAGCGGTTTATTGTGTTATTAGACAAGAAGCTGTGTTGCCATGACTACA
TCTCTAGGGAATTTCTTAGTCTATTACTT
Primers used for PCR WS05 - 8474, show primer details
   
Sequence Name 30-K019823-022-333-E05C-8409
GenBank Accession LN499532 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K019823-022-333-E05C-8409
GATCAATGTTGAATTTCTTGCAAAAAGGTACCCATTTCTTTGAGAACTCAGCCGTTTCAG
AAAGGGCTTCAAAAGTTAGCATAGCTGAACCGTCATCCGAAACGTAACACGCAACTTTAT
CCACTGGGTAGTCCACAGCAAGGATTGAGAGAACTGTGTTTGCTGTTACAAGAGGAGGCT
CTTTCATCGGGTCCACCGTACTAACAAACACATCCACGGGAGCTAGCTGTGATGGTTCAC
CATCTCGATCGTATCTATAATTAAGGATTAAAACCAAAAAGTCAAATGCAAGTAATGTAA
ACCAGATGTAGATGATCATCAACTTAGAAAGACCAACCTTAAAGCAAGCCTGTCAAGAAA
AGTCTCCCTGTTAATGGGATACCATTTTGGGAACTGATCAAGAAGCCAAGAAAAGGCAAA
CCAGATCTCACAAATAACTGAGGTCAACCACAGGGCATATGCATCTTTCACGGGATGAGT
TGTACGATAATGCAAGAAGACTCCCAAAATGATGAGCCG

Last Updated on 06.12.2018 16:11