Sequences from confirmation amplicon


Confirmation sequences for line 343B05 and hit At1g05120 are shown.
Line ID 343B05
Locus At1g05120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1472700
Primers used for PCR HP24 - 8474, show primer details
   
Sequence Name 35-K019550-022-343-B05C-HP24
GenBank Accession LN501643 [GenBank]
Sequencing primer gene specific primer, HP24: 5'-TTGGTTGATGAGAACAAAAGTGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K019550-022-343-B05C-HP24
GAATGTGGTCTCTGCCACGATCCGGCCGAGGACTACGTTGTAAGTATTTTAAGTCAATCT
ATTGCTATTTCGTAATATCTTGACTCTTGTTTCTCAGTAACTGTATCTTCTGGAAACAGC
TGCTTTCATTTGACACTGTTTAAGAATCGTCAGATTACTATTGTTGGACTTACTTTTCCC
TTTAATATTCTTCTCTGGTTCTTTTACTTTGAAGGTGACTTCTTGTGCACATGTGTTCTG
TAAAGCTTGTTTGATTGGTTTCTCCGCGTCCCTGGGAAAAGTCACCTGCCCGACATGCTC
GAAACTACTGACTGTGGATTGGACTACCAAAGCTGACACAGAGTATAAATGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT
   
Sequence Name 36-K019550-022-343-B05C-8409
GenBank Accession LN502042 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K019550-022-343-B05C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACATTTATACTCTGTGTCAGCTTTGGTAGT
CCAATCCACAGTCAGTAGTTTCGAGCATGTCGGGCAGGTGACTTTTCCCAGGGACGCGGA
GAAACCAATCAAACAAGCTTTACAGAACACATGTGCACAAGAAGTCACCTTCAAAGTAAA
AGAACCAGAGAAGAATATTAAAGGGAAAAGTAAGTCCAACAATAGTAATCTGACGATTCT
TAAACAGTGTCAAATGAAAGCAGCTGTTTCCAGAAGATACAGTTACTGAGAAACAAGAGT
CAAGATATTACGAAATAGCAATAGATTGACTTAAAATACTTACAACGTAGTCCTCGGCCG
GATCGTGGCAGAGACCACATTCTTGCTCACTTTTGTTC

Last Updated on 06.12.2018 16:11