Sequences from confirmation amplicon


Confirmation sequences for line 345F09 and hit At3g07940 are shown.
Line ID 345F09
Locus At3g07940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2529512
Primers used for PCR 10gz - 8474, show primer details
   
Sequence Name 49-K112309-0022-345-F09C-10gz
GenBank Accession LN506890 [GenBank]
Sequencing primer gene specific primer, 10gz: 5'-CAGCACAATATTTATTCCCAGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K112309-0022-345-F09C-10gz
AACAAACAAACAGGACATGAGATTGTCTCCAACTAACACTTAAAACATATCTTAAGAATG
TTTATCTCCATCATTAAGTAATAATTACCAGAAGAAGATGTTTGCAAATCTTCAACCCTA
AGAGGAGTCCATTTGGGTGTCTCAGAACATAAAAGTTCATATAAGCAAGCATGCGACCCT
ATAAAACAAAAAGGCAAAAATTTGAAACCTTTACAAGTTTAAAAACTTGGTGGATAATAA
TTATTAAAGGATTGTGTAGGATTTTACCTGAAACTTCAACAGGATCAACATTTTCTTGGC
CGAGAGACATTTCGAAGCAGAGCCGAGACAAGAAAGAACAATATGGACGGTCACTTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 33-K112308-0022-345-F09C-8409
GenBank Accession LN501054 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K112308-0022-345-F09C-8409
CATTTCAGTGACCGTCCATATTGTTCTTTCTTGTCTCGGCTCTGCTTCGAAATGTCTCTC
GGCCAAGAAAATGTTGATCCTGTTGAAGTTTCAGGTAAAATCCTACACAATCCTTTAATA
ATTATTATCCACCAAGTTTTTAAACTTGTAAAGGTTTCAAATTTTTGCCTTTTTGTTTTA
TAGGGTCGCATGCTTGCTTATATGAACTTTTATGTTCTGAGACACCCAAATGGACTCCTC
TTAGGGTTGAAGATTTGCAAACATCTTCTTCTGGTAATTATTACTTAATGATGGAGATAA
ACATTCTTAAGATATGTTTTAAGTGTTAGTTGGAGACAATCTCATGTCCTGTTTGTTTTG
TTTTAGATCCCAGAGATCGGTTGGAGAAGTTGCTGAAGCAACCTGGGAATAAATATTGTG
CTG

Last Updated on 06.12.2018 16:11