Sequences from confirmation amplicon


Confirmation sequences for line 346C11 and hit At2g48160 are shown.
Line ID 346C11
Locus At2g48160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19694260
Primers used for PCR SP13 - 8474, show primer details
   
Sequence Name 46-K103582-0022-346-C11C-SP13
GenBank Accession LN505841 [GenBank]
Sequencing primer gene specific primer, SP13: 5'-TGCAGATGAATCCACAGTATTAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K103582-0022-346-C11C-SP13
TACGGCTGGAAGATCAGACTCACTAGTTAGCAGCTTCAGGACCTCAGGGCACACAGCTTC
TGGAACGCTAGCTGTCTGAGCTTCGCCAGCTGGCAAACTGCTAACTTGATTATATTGGGG
TCCAGCTGATTTATCATCTGGTGTCTTGCTAAGCTCATTCCACCCCATAGCATATTCTCT
CTCTCTCACAGTCTGAACGCAAGCCTCAGATGGAGACATTCCATCATGAGATTTCTCCAC
GGGAGATGGTGCTACATTGACCATGTCCCCTGAGAGCTTAGCAGACACTTCAAAGAATTT
AGATTCTTCAACTGCAAAAGTATCATGACTAGAAATGATATCAGAAGGGCTTGTCTGTGC
TGTTGCACTGACCACAGTGTCCTTGGGTGATCTTTCTTCCGCCTGCAAAGAGCTATATGT
GGGGACATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCC
   
Sequence Name 62-K103583-0022-346-C11C-8409
GenBank Accession LN510737 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K103583-0022-346-C11C-8409
CACATATAGCTCTTTGCAGGCGGAAGAAAGATCACCCAAGGACACTGTGGTCAGTGCAAC
AGCACAGACAAGCCCTTCTGATATCATTTCTAGTCATGATACTTTTGCAGTTGAAGAATC
TAAATTCTTTGAAGTGTCTGCTAAGCTCTCAGGGGACATGGTCAATGTAGCACCATCTCC
CGTGGAGAAATCTCATGATGGAATGTCTCCATCTGAGGCTTGCGTTCAGACTGTGAGAGA
GAGAGAATATGCTATGGGGTGGAATGAGCTTAGCAAGACACCAGATGATAAATCAGCTGG
ACCCCAATATAATCAAGTTAGCAGTTTGCCAGCTGGCGAAGCTCAGACAGCTAGCGTTCC
AGAAGCTGTGTGCCCTGAGGTCCTGAAGCTGCTAACTAGTGAGTCTGATCTTCCAGCCGT
ACAATACTGTCAAGTTGCTAAAATTGAACCCTCCATGGATCCTAATACTGT

Last Updated on 06.12.2018 16:11