Sequences from confirmation amplicon


Confirmation sequences for line 348A07 and hit At1g20890 are shown.
Line ID 348A07
Locus At1g20890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7266692
Primers used for PCR 04ag - 8474, show primer details
   
Sequence Name 14-K106400-0022-348-A07C-8409
GenBank Accession LN492519 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K106400-0022-348-A07C-8409
GCATTTACAATTGGTTCTTGTGCTTGTTAAAAATCTCATCCTTTTTTGTTATGATAACAG
ATACCGGCTCTATTTTCGCTGCAATTACTGAAGAACGGTGTAATGGGTTTGAAGGTTAAC
CATATGAGGAGATTACTAAACACTGAGAAGTTGATCTTCAAAACAGAAAGTATTGAAGAA
GTTGATAACAAGGTATTGCCATCTTCTTCAGTTAGTTCCTAAAACTTAGAAAGAAGAACG
AATACAAGTTTAGGAGTTTGTTTCTGATTTAGGAGGGATTGTATGTTTTGGTGACGGTGG
AACCTGAAGGAATCGTGGCTATACCAAATGTTAAAGAAAGACCGTCCATCATTTACAACA
TAGGCAAGCTTTTTTTTCTACTTACTATCTCATCAATCTTCTTCTTTTCACAACATAAGA
ACTTGTATTAAGTGTAGTGCTTCTTCTAGAGTCTGAAAACTCTCATGTTGACAGTTTGTG
AAGAACAGCTCTTAGGCATCCCTCACTCAAGCTGGTCAGTGGTGGTTTTAGTAGTTTTGT
GTCTCGTTGTCGCGTTGATTCTTCCCCGGTTCCTCCCATC
   
Sequence Name 30-K106401-0022-348-A07C-04ag
GenBank Accession LN499731 [GenBank]
Sequencing primer gene specific primer, 04ag: 5'-GATCAGAAGATAAGATGGGAGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K106401-0022-348-A07C-04ag
AAACTACTAAACCACCACTGACCAGCTTGAGTGAGGGATGCCTAAGAGCTGTTCTTCACA
AACTGTCAACATGAGAGTTTTCAGACTCTAGAAGAAGCACTACACTTAATACAAGTTCTT
ATGTTGTGAAAAGAAGAAGATTGATGAGATAGTAAGTAGAAAAAAAAGCTTGCCTATGTT
GTAAATGATGGACGGTCTTTCTTTAACATTTGGTATAGCCACGATTCCTTCAGGTTCCAC
CGTCACCAAAACATACAATCCCTCCTAAATCAGAAACAAACTCCTAAACTTGTATTCGTT
CTTCTTTCTAAGTTTTAGGAACTAACTGAAGAAGATGGCAATACCTTGTTATCAACTTCT
TCAATACTTTCTGTTTTGAAGATCAACTTCTCAGTGTTTAGTAATCTCCTCATATGGTTA
ACCTTCAAACCCATTACACCGTTCTTCAGTAATTGCAGCGAAAATAGAGCCGGTATCTGT
TATCATAACAAAAAAGGATGAGATTTTTAACAAGCACAAGAACCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11