Sequences from confirmation amplicon


Confirmation sequences for line 349B01 and hit At3g47980 are shown.
Line ID 349B01
Locus At3g47980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17711725
Primers used for PCR 13nt - 8474, show primer details
   
Sequence Name 75-K114626-0022-349-B01C-13nt
GenBank Accession LN513286 [GenBank]
Sequencing primer gene specific primer, 13nt: 5'-ACGTAAAGGTGTAGTGGCCTAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K114626-0022-349-B01C-13nt
CATAGAACAAATAGAAAATTTACCCCTACTCGAGACCTACTACTATCCTAATCACTATTT
AATTGATTTTTATTTATGTTTCATTCCAAAATTCTATATTAAGATTAGGATATCCGCTGT
GCGCTAATTTTTTAATTTATTTTTGCTAGGATTATATTAGAAAATTATTCTCAATATTTT
AGTGATGAAATTTCTATATTTCCAAAAAATAGAATTGAATTACCAGGCGGATGGTTGGCA
CGAGTGATGACCATGAACGCGATGGAAGCGGCGAGAGCGGTACTGCGGGCTAGCCAGCTT
GGCCCAAAGGCGGAGAACGCCAACACTCCAATGGCTGCACAACCTATTTGAGCCGTGAAC
ATATTGTATTTCTGAAATAAATACACATGCACCCATCAACAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 75-K114625-0022-349-B01C-8409
GenBank Accession LN513285 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K114625-0022-349-B01C-8409
TACATTGTTGATGGGTGCATGTGTATTTATTTCAGAAATACAATATGTTCACGGCTCAAA
TAGGTTGTGCAGCCATTGGAGTGTTGGCGTTCTCCGCCTTTGGGCCAAGCTGGCTAGCCC
GCAGTACCGCTCTCGCCGCTTCCATCGCGTTCATGGTCATCACTCGTGCCAACCATCCGC
CTGGTAATTCAATTCTATTTTTTGGAAATATAGAAATTTCATCACTAAAATATTGAGAAT
AATTTTCTAATATAATCCTAGCAAAAATAAATTAAAAAATTAGCGCACAGCGGATATCCT
AATCTTAATATAGAATTTTGGAATGAAACATAAATAAAAATCAATTAAATAGTGATTAGG
ATAGTAGTAGGTCTCGAGTAGGGGTAAATTTTTCTATTTGTTCTATGCAATGGATCAAAT
CATAGATTCTTACTATCATGCATTTTAGGCCACTAANCCTTTAC

Last Updated on 06.12.2018 16:11