Sequences from confirmation amplicon


Confirmation sequences for line 351C04 and hit At3g58030 are shown.
Line ID 351C04
Locus At3g58030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21486692
Primers used for PCR GD51 - 8474, show primer details
   
Sequence Name 74-K033713-022-351-C04C-8409
GenBank Accession LN513010 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K033713-022-351-C04C-8409
GCCTTTACAATTGAAATGAGTTTATAACAGCTGCAATGCTTGAGAACGAGTCTCTATCAT
CAACCACAACAGGAGCATGATGGTTTTGCTCTTGGTGGTTCCTCCCCAACGGATGAGTCC
TAAAATACGCATCCACAAGCCTCTCAGCTGAACTCAACGCAGAAGTGAAAGTCGAGATCC
TTGCAACTCGGTGCGACTGTAACTGTCTCCTGATCAAGAGAGGATGGAATCTCGTGTTGC
TTTCTCCTTCAAGATCAGGAGCAATGTTTGGATTTAGATCAATATCCTCTGATGCTGCGA
CAATGGCTGCTGCTGCAGCACTAGCCTGGTTCTGCTCTGATCTAACTCCCCTAGATGTCA
TCAACCGGTTAAGGATCGAATTGGCTCGATCGTTGACTCTTTCTGATGCCTCTCGGTTAC
TAAAATCAGGGACTGGGGTTGAATCCCTGTCAAACCTATTCTGTATACGTCTAATCATTT
CTTCCATTGGTATGTTAAAAGG
   
Sequence Name 90-K033713-022-351-C04C-GD51
GenBank Accession LN515000 [GenBank]
Sequencing primer gene specific primer, GD51: 5'-GCCTTTTACAATACCAATGGAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K033713-022-351-C04C-GD51
GTTTGACGGGATTCAACCCCAGTCCCTGATTTAGTAACCGAGAGGCATCCGAAAGAGTCA
ACGATCGAGCCAATTCGATCCTTAACCGGTTGATGACATCTAGGGGAGTTAGATCAGAGC
AGAACCAGGCTAGTGCTGCAGCAGCAGCCATTGTCGCAGCATCAGAGGATATTGATCTAA
ATCCAAACATTGCTCCTGATCTTGAAGGAGAAAGCAACACGAGATTCCATCCTCTCTTGA
TCAGGAGACAGTTACAGTCGCACCGAGTTGCAAGGATCTCGACTTTCACTTCTGCGTTGA
GTTCAGCTGAGAGGCTTGTGGATGCGTATTTTAGGACTCATCCGTTGGGGAGGAACCACC
AAGAGCAAAACCATCATGCTCCTGTTGTGGTTGATGATAGAGACTCATTCTCAAGCATTG
CAGCTGTTATAAACTCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11