Sequences from confirmation amplicon


Confirmation sequences for line 352H03 and hit At3g22410 are shown.
Line ID 352H03
Locus At3g22410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7934339
Primers used for PCR 15ve - 8474, show primer details
   
Sequence Name 40-K118051-0022-352-H03C-15ve
GenBank Accession LN503863 [GenBank]
Sequencing primer gene specific primer, 15ve: 5'-GATATCTAGCGGTTCGTCGTAGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K118051-0022-352-H03C-15ve
AAAGGACGAACACCCTGACAATTGGTACGAACAAACAAAACGTACTTTTGAGCTTATCCA
TACTAATCACAATTAGAGAATTAATAATATGTAATTAATGGGTTTAGGGTTATAGAATAA
TTACCTTCCAAAGGTAAGAGAAAAAAGAGGGAGGGTCGATGATAAAGGCTTTGTAAAGTC
GGCATGGGTAATTATCTGCGATGATTTTTAGGGTCGCCAAAAGCAAATTCGCAAATGCAG
AGGATGATCTGAAAAAGCCTGTTTTTTTTGTATGGTATTAAGAAAAAGGAATATTAATCG
ATTTTGTACAAATATTTAAATTGTCTAATCGGGTTTGAAATTTTTTTGAAGGTGTATTTT
TGCAGAGAATAAAATGTAGATGTCCGCAGCGATTATTAT
   
Sequence Name 04-K118050-0022-352-H03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K118050-0022-352-H03C-8409
CCACTAACATCACTTGCTTATTATTTTGTTAAGTAGACCATAGTGGAAATATGTTTGTTT
TGCTAAATAAGTCTTAGGTTTGGTGAATGTGTTGCACCCGGTGAAA

Last Updated on 06.12.2018 16:11