Sequences from confirmation amplicon


Confirmation sequences for line 356A12 and hit At3g11450 are shown.
Line ID 356A12
Locus At3g11450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3605924
Primers used for PCR 10lc - 8474, show primer details
   
Sequence Name 50-K113934-0022-356-A12C-10lc
GenBank Accession LN507241 [GenBank]
Sequencing primer gene specific primer, 10lc: 5'-GAATCTGCACAACAACAAAAGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K113934-0022-356-A12C-10lc
TCTTACGCAAGACGAAATCGTCTTAGAACTCTCTCAGCTCCTCTCGTGGCTCAGCGTCTG
CTTGACATCTCTGAGGAAGATATAGAGAATCTATGCATGTCACTTAACACCGAGCAGTTG
CAGAATCTATGTGATAAAATGGGAAACAAAGAAGGACTAGAATTGGCAAAGGTGATCAAA
GATGGATGCAACAGTAGTAGAAATGATGAAGCCGAATCTAAAGAAAAAGTAAGTAAGAAG
ACCAACGGTGGCACAGAGCCTACAACCCGAGTTTCACAGTTAGATAGTAGCACTCAGAAG
AAACAACCATGGAGCAAGGAAGAGATTGATATGCTGAGAAAAGGAATGATAAAATATCCA
AAGGGGACATCGCGGAGATGGGAAGTTATTTCATAATCATCATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 15-K113933-0022-356-A12C-8409
GenBank Accession LN493057 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K113933-0022-356-A12C-8409
ATTTACATTGAATATGATGATTATGAAATAACTTCCCATCTCCGCGATGTCCCCTTTGGA
TATTTTATCATTCCTTTTCTCAGCATATCAATCTCTTCCTTGCTCCATGGTTGTTTCTTC
TGAGTGCTACTATCTAACTGTGAAACTCGGGTTGTAGGCTCTGTGCCACCGTTGGTCTTC
TTACTTACTTTTTCTTTAGATTCGGCTTCATCATTTCTACTACTGTTGCATCCATCTTTG
ATCACCTTTGCCAATTCTAGTCCTTCTTTGTTTCCCATTTTATCACATAGATTCTGCAAC
TGCTCGGTGTTAAGTGACATGCATAGATTCTCTATATCTTCCTCAGAGATGTCAAGCAGA
CGCTGAGCCACGAGAGGAGCTGAGAGAGTTCTAAGACGATTTCGTTCTTTGCGTAAGAGC
TTCTTCTCTCTTTCCTTAGTTTTCTTTTGTTGTTGTGCAGATT

Last Updated on 06.12.2018 16:11