Sequences from confirmation amplicon


Confirmation sequences for line 356D09 and hit At4g02970 are shown.
Line ID 356D09
Locus At4g02970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1318832
Primers used for PCR GE31 - 8474, show primer details
   
Sequence Name 96-K033713-022-356-D09C-GE31
GenBank Accession LN515367 [GenBank]
Sequencing primer gene specific primer, GE31: 5'-CAAGAGAGGGGTGAGTTAAATCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>96-K033713-022-356-D09C-GE31
TTGACCGAAACTAAGTCATTCAAGTGGAACCGTGTTGCTGATGGCGATACAATTGAGATT
ATCCGTAGACTCGTGCCTTTTTGCAGTCTCCTCTTTTCTCATACCTAGTTTTTAGTCTTT
CTTCAAGTTTTCTTGCTTTGTTCAAAGTTCATAGCTTTCTTCAAATATGCTAAGACATGT
AAGTTCATAGCTTTCTTCTTGAGTTACATCTTTTCGAAATTAGCTCTGTACATTCTCTAG
AAAACATTACGATCGGGTGGTCTAGTTCACATTTCTTTTGGTATTTTTCAATGTCTAGTT
ACAGCAACAAGTATGAAGATCAAATGTTGAATCTTTGTTGATAAAACAGGGCAGAAAGAC
AGTAAAGTCTCTTGCTTTACTCAAAAACTTAACCAAAAAAACAATCAAAATACTAATTGC
GTCAAAACACTCTGCAACTAATGAACGATAACGTGAAAGAACTGAGAAACGAGTTGATGA
AGATTGAAGAGGAAGGTGAGTAAAAGCTGCCTCAAGAAGGGTATTTTCTTATACACAAAA
ACAGAGTATTGAAAGAGAATCAGTCATTCTCTTGCTAAAGAAAATGGCTTCATGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGA
AAGCAATAAACAAATTATTCTAATTCGGAGATCTTTATNTCGACGTGTCTACATC
   
Sequence Name 80-K033713-022-356-D09C-8409
GenBank Accession LN513900 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K033713-022-356-D09C-8409
GCCATTTACTGAAGCCATTTTCTTTAGCAAGAGAATGACTGATTCTCTTTCAATACTCTG
TTTTTGTGTATAAGAAAATACCCTTCTTGAGGCAGCTTTTACTCACCTTCCTCTTCAATC
TTCATCAACTCGTTTCTCAGTTCTTTCACGTTATCGTTCATTAGTTGCAGAGTGTTTTGA
CGCAATTAGTATTTTGATTGTTTTTTTGGTTAAGTTTTTGAGTAAAGCAAGAGACTTTAC
TGTCTTTCTGCCCTGTTTTATCAACAAAGATTCAACATTTGATCTTCATACTTGTTGCTG
TAACTAGACATTGAAAAATACCAAAAGAAATGTGAACTAGACCACCCGATCGTAATGTTT
TCTAGAGAATGTACAGAGCTAATTTCGAAAAGATGTAACTCAAGAAGAAAGCTATGAACT
TACATGTCTTAGCATATTTGAAGAAAGCTATGAACTTTGAACAAAGCAAGAAAACTTGAA
GAAAGACTAAAAACTAGGTATGAGAAAAGAGGAGACTGCAAAAAGGCACGAGTCTACGGA
TAATCTCAATTGTATCGCCATCAGCAACACGGTTCCACTTGAATGACTTAGTTTCGGTCA
ATGGTAACTCACTGCTTACCAAGTGAAACGCTTCGTGAGGCAGATTTAACTCACCCTTCT
CT
Primers used for PCR GE32 - 8474, show primer details
   
Sequence Name 01-K033715-022-356-D09C-GE32
GenBank Accession LN486467 [GenBank]
Sequencing primer gene specific primer, GE32: 5'-CTCTTCTTCTTGCATTTGCTTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K033715-022-356-D09C-GE32
TCTCATCTAATCTGTCTCTTCATATTACTATTGGGCTTTTGGTTAGTCTATACTGGGACT
CAAAATAAGGCTTTGGCCCACATCATAAAAGATACATTCACAAATCAAAACTAATTTTCA
GAGTCTTTTGTCCCACATCGGTCAATCTACTCGTTTTGTGTTTGTTTATATATTACACGA
AACGATGTATTCAACGTCGAGCTAAGTAACAATAGCTTGTAACCCATGTGGGGACATTAA
GATGGTGGGACACTGGTTCGGTTCCTCGGATCGGTTCTGATGTGGGCATGTTTCTGAGCT
GCCCAGTCCAAGCTGTGAGTAATTAGTGTTGAGTTGAGCGAAGGCTTGGCTCATGCAGGT
TCTAGAGTCGGAGGGTAATGCGTGAGGCTGGCTTCACAGAGCAGCGACTACTTCCCGCTT
ACAGCAGTGGACGGATCACGGTTTAGCGTCGCTCAGAACCACTATGGCCTGCTGGTCCGA
TCTCATATGAACCACCATTTTTTTCCCTCTTTTGATTTTTGTTTTCCAAAACTTTCTACG
CTTTTTGTTTATATTTTAAGAGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTAT
TTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
ACATTC
   
Sequence Name 81-K033715-022-356-D09C-8409
GenBank Accession LN514016 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K033715-022-356-D09C-8409
AACAAAAGCGTAGAAAGTTTTGGAAAACAAAAATCAAAAGAGGGAAAAAAATGGTGGTTC
ATATGAGATCGGACCAGCAGGCCATAGTGGTTCTGAGCGACGCTAAACCGTGATCCGTCC
ACTGCTGTAAGCGGGAAGTAGTCGCTGCTCTGTGAAGCCAGCCTCACGCATTACCCTCCG
ACTCTAGAACCTGCATGAGCCAAGCCTTCGCTCAACTCAACACTAATTACTCACAGCTTG
GACTGGGCAGCTCAGAAACATGCCCACATCAGAACCGATCCGAGGAACCGAACCAGTGTC
CCACCATCTTAATGTCCCCACATGGGTTACAAGCTATTGTTACTTAGCTCGACGTTGAAT
ACATCGTTTCGTGTAATATATAAACAAACACAAAACGAGTAGATTGACCGATGTGGGACA
AAAGACTCTGAAAATTAGTTTTGATTTGTGAATTTATCTTTTATGATGTGGGCCAAAGCC
TTATTTTGAGTCCCATTATAGACTAACCAAAAGCCCAATAGTAATATTGAAGAGACAGAT
TTGAGATGGAAGAAGAGAGAAAGGGGCAAAGAAAGCAAATGCAGAAAGAAGA

Last Updated on 06.12.2018 16:11