Sequences from confirmation amplicon


Confirmation sequences for line 361D06 and hit At1g63910 are shown.
Line ID 361D06
Locus At1g63910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23721271
Primers used for PCR RS02 - 8474, show primer details
   
Sequence Name 47-K018493-022-361-D06C-RS02
GenBank Accession LN506043 [GenBank]
Sequencing primer gene specific primer, RS02: 5'-ACTAGCTATATGAGCCCACCTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K018493-022-361-D06C-RS02
AAAAACATAAGTTTAATAAGAAAATATGTATGTATCACATTTTGAGAATGTTCAATGAAG
TAACTTAAAGTGTACTACTTAATAGCTCGACCAAGTAGACAAAAGTAAACGAGCTAGATG
AATGAAACTACACGTTAAAGATTCTTTGGTCATCGACAGTAGGCTAGTCGTTGCTTCAAA
CCGTGGCCAAACTAAACATATAATTGTTTAAATTTAAAATATAACATGATAAACTGAATA
TAAGTATAAAATGTACTATGTATGTATCCCAACCCAACAAACTTATTATTGAAAATGGTA
TCAGTATTGAAAATCTTATAAGACTTCCTGTAGCGGTCTATAATAATACACTATCTTGAA
TTGATAATATATAAAAATTTAAGTCGAGTTAGTAAAAATGGAGACCTGTTTCCCACAACT
CCATGAAGGCTTATGATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCT
   
Sequence Name 48-K018493-022-361-D06C-8409
GenBank Accession LN506369 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K018493-022-361-D06C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGATCATAAGCCTTCATGGAGTTGTGGG
AAACAGGTCTCCATTTTTACTAACTCGACTTAAATTTTTATATATTATCAATTCAAGATA
GTGTATTATTATAGACCGCTACAGGAAGTCTTATAAGATTTTCAATACTGATACCATTTT
CAATAATAAGTTTGTTGGGTTGGGATACATACATAGTACATTTTATACTTATATTCAGTT
TATCATGTTATATTTTAAATTTAAACAATTATATGTTTAGTTTGGCCACGGTTTGAAGCA
ACGACTAGCCTACTGTCGATGACCAAAGAATCTTTAACGTGTAGTTTCATTCATCTAGCT
CGTTTACTTTTGTCTACTTGGTCGAGCTATTAAGTAGTACACTTTAAGTTACTTCATTGA
ACATTCTCAAAATGTGATACATACATATTTTCTTATTAAACTTATGTTTTTTGTTTTTTT
CAGGTGGGCTCA

Last Updated on 06.12.2018 16:11