Sequences from confirmation amplicon


Confirmation sequences for line 367C04 and hit At2g39675 are shown.
Line ID 367C04
Locus At2g39675 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16537369
Primers used for PCR LD15 - 8474, show primer details
   
Sequence Name 75-K030473-022-367-C04C-8409
GenBank Accession LN513164 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K030473-022-367-C04C-8409
GAAAACGAACAATTTTCGGGTAGGAAATTTTTGAAAATATAGATTTACGGATTCGACATC
ATTCACTGAGTAACAACAATACTTTAAGTTAGTTCCCTCGTCATAATTATTAACCAAAGT
AAATATCATCACACCTATCGTCAAAGTCGTCACATCTCAAACGTGCTTCAATCTCTTCAT
CCTTGGTTGCACACTGACCAACCAACCCTCACGACGATTTTTCCTTTCCGTGATTGAGCC
ATGTCTCCTCTTCAAACCAAAGTTGAACAGAGCCATCCAATGGCAAAACCGGAAATTTGT
GCAATCCAGGACATCAACAAGCGACAGCTTCTGCATCTGGCTGAGACTCTTGACTATCCG
TCTCAGTAAGCAAAGGGCTTTTCTGAAACTTATTCCT
   
Sequence Name 76-K030473-022-367-C04C-LD15
GenBank Accession LN513320 [GenBank]
Sequencing primer gene specific primer, LD15: 5'-GTGGTCAGCTGAGGAATAAGTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K030473-022-367-C04C-LD15
GAACAAGGGAAACTAACTTAAAGTATCGTTGTTACTCAGTGAAAGAAGTCAAATCCGTAA
ATCTATATTTCAAAATTCCTACCCGAATTGTCATTTTCACTTTACGATGTGGTGTTCTAT
TTTCATCTCTTGACCCAGAATTATATGATGGTCGATACAGTTGTAAAGTTTTAATTGATC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTA
TTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTC

Last Updated on 06.12.2018 16:11