Sequences from confirmation amplicon


Confirmation sequences for line 383G06 and hit At5g05950 are shown.
Line ID 383G06
Locus At5g05950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1788846
Primers used for PCR KP70 - 8474, show primer details
   
Sequence Name 81-K034064-022-383-G06C-KP70
GenBank Accession LN514018 [GenBank]
Sequencing primer gene specific primer, KP70: 5'-CAGAAGCCAAACCAAGAGATTTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K034064-022-383-G06C-KP70
TCAAACATAGAAATCATTCTCATTTCCATCATATCATCATCATACAAACATAACCCTAAT
TGCATCAACCATGGAATCAAAAACCATGTCTCTAACGAGTAAAAGCATATAACATAAGAG
AAACATAGATGAAAAGTGCAGAAGGAACCAGAATCACAAGCGGCACAACAGCTGCATAAG
TATGACCACTAGCACCACAGCCCAAAGTCCCAAGCTTGATCTGAACAACATTAATCAAAG
CCAACATGAGAAACCCACATCCACAAACCGATCCCAAACCGGATGTAACCATCCCAAATC
TAAGAGCCGTCTTGTTCACATAGTAAACCACAGGATCGATTCGAGTAGAGATGTGAAACG
AAGAAGCTATGTTGAGCCTCATTGCTTGTTTGAGACCTAGAGCGATGAGACTTGAGAATA
GGAATGATGCGAAAGAGTACACATGGAAGGCGACGAGATTCTCAGCCATACGAGCTGTGG
GGACGCAATTAGGGTCGGTTACGAGGCTGTTGTCTGGATCGGTAGGGTTCCAAGCTAATC
CGATGAATACGGCGAGTGTGAAGAGTGAGTTCACGTTGACGATTCCGAGAAATGCATGCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTACA
   
Sequence Name 14-K034064-022-383-G06C-8409
GenBank Accession LN492411 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K034064-022-383-G06C-8409
GCATGCATTTCTCGGAATCGTCAACGTGAACTCACTCTTCACACTCGCCGTATTCATCGG
ATTAGCTTGGAACCCTACCGATCCAGACAACAGCCTCGTAACCGACCCTAATTGCGTCCC
CACAGCTCGTATGGCTGAGAATCTCGTCGCCTTCCATGTGTACTCTTTCGCATCATTCCT
ATTCTCAAGTCTCATCGCTCTAGGTCTCAAACAAGCAATGAGGCTCAACATAGCTTCTTC
GTTTCACATCTCTACTCGAATCGATCCTGTGGTTTACTATGTGAACAAGACGGCTCTTAG
ATTTGGGATGGTTACATCCGGTTTGGGATCGGTTTGTGGATGTGGGTTTCTCATGTTGGC
TTTGATTAATGTTGTTCAGATCAAGCTTGGGACTTTGGGCTGTGGTGCTAGTGGTCATAC
TTATGCAGCTGTTGTGCCGCTTGTGATTCTGGTTCCTTCTGCACTTTTCATCTATGTTTC
TCTTATGTTATATGCTTTTACTCGTTAGAGACATGGTTTTTGATTCCATGGTTGATGCAA
TTAGGGTTATGTTTGTATGATGATGATATGATGGAAATGAGAATGATTCTATGTTTGATA
TGGTTCTTTGTAAATCTCTTGTTGGGG

Last Updated on 06.12.2018 16:11