Sequences from confirmation amplicon


Confirmation sequences for line 384G02 and hit At5g06150 are shown.
Line ID 384G02
Locus At5g06150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1861479
Primers used for PCR MZ56 - 8474, show primer details
   
Sequence Name 07-K025300-022-384-G02C-8409
GenBank Accession LN489102 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K025300-022-384-G02C-8409
ATTTCCCGGACATGAAGCCATTTACAATTGAATATATCAATTGAATTAAGAACGACGATA
TCAACGATAAACCCATTAAAAGACGCGAAGAACAAACCTCTGACTTGTTCAGGTACGTTT
GCTCTCGTCGCCATGATCGCTCTCCCAATGATTCTTACTCTTCTTGTTTCTTAGTCTTGA
TATTTCTCTTTCGTAAAGAGTCTCTGCGATTGTGACGAAGATTGGAAGCGAGCGCTGCTT
ATGAAGAGGAGGAGGAGCCAACGGTTCAATTCCAACGGTCATATTTTTGGGGATTTCATC
TGTTGCCGTTAGATGATTAAATTTTAGATCTAACGGTGGATAGGTTAGTCTTCGAAGTTG
GAATGCGCTGGCACTGGATAATTTTACACTAGTCATCGAATGAGGCCGTTGGACTGTAAA
TAAAAAAGGTAAAGCCCATCC
   
Sequence Name 08-K025300-022-384-G02C-MZ56
GenBank Accession LN489566 [GenBank]
Sequencing primer gene specific primer, MZ56: 5'-AATAAAAGTTGGGATGGGCTTTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K025300-022-384-G02C-MZ56
TTTTTTATTTCAGTCCAACGGCCTCATTCGATGACTAGTGTAAAATTATCCAGTGCCAGC
GCATTCCAACTTCGAAGACTAACCTATCCACCGTTAGATCTAAAATTTAATCATCTAACG
GCAACAGATGAAATCCCCAAAAATATGACCGTTGGAATTGAACCGTTGGCTCCTCCTCCT
CTTCATAAGCAGCGCTCGCTTCCAATCTTCGTCACAATCGCAGAGACTCTTTACGAAAGA
GAAATATCAAGACTAAGAAACAAGAAGAGTAAGAATCATTGGGAGAGCGATCATGGCGAC
GAGAGCAAACGTACCTGAACAAGTCAGAGGTTTGTTCTTCGCGTCTTTTAATGGGTTTAT
CGTTGATATCGTCGTTCTTAATTCAATTGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAA

Last Updated on 06.12.2018 16:11