Sequences from confirmation amplicon


Confirmation sequences for line 399D03 and hit At2g35035 are shown.
Line ID 399D03
Locus At2g35035 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14764938
Primers used for PCR HM02 - 8474, show primer details
   
Sequence Name 15-K028846-022-399-D03C-8409
GenBank Accession LN492835 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K028846-022-399-D03C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATTTCCCGGGTATTCTCTCTCTAC
GTAGAGTTCACAGTTTCGAATTTTCAATCTTTAGTTATTAAAGTTCCGGCGACACTGAAC
TTTCAGGCAGCTCCTGCTGGAACTGACGTTGTCTGGATTTACAGTATCACTTATGGTGGC
GGCATTGTCTCTGTATTTCCCTATTTCTCTGTCTCTTTATTAAAGAATTTGCCATCGATG
CGCTTGAATCAATCTAGTTCTTGTTCAAATTATGTAGGGCGATTCCATTTCATGTGAATT
CACAATTGGTGATGGATGCACTGCAGTTATTACAACGCAGTCTTCTACT
   
Sequence Name 16-K028846-022-399-D03C-HM02
GenBank Accession LN493304 [GenBank]
Sequencing primer gene specific primer, HM02: 5'-CAAGATCCATCAACAGAAAGTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K028846-022-399-D03C-HM02
CCACGTAGGATGAAATTAGTGTCCATTGCAAAGAGAGAGAAAAGCCCTAAATTGATTCAA
CCTTAGTAGAAGACTGCGTTGTAATAACTGCAGTGCATCCATCACCAATTGTGAATTCAC
ATGAAATCGAATCGCCCTACATAATTTGAACAAGAACTAGATTGATTCAAGCGCATCGAT
GGCAAATTCATTAATAAAGAGACAGAGAAATAGGGAAATACAGAGACAATGCCGCCACCA
TAAGTGATACTGTAAATCCAGACAACGTCAGTTCCAGCAGGAGCTGCCTGAAAGTTCAGT
GTCGCCGGAACTTTAATAACTAAAGATTGAAAATTCGAAACTGTGAACTCTACGTAGAGA
GAGAATACCCGGGAAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAAC
GACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGG
AAATCTTTATTTCGACGTGTCTACATTCACGTCCCCATGG

Last Updated on 06.12.2018 16:11