Sequences from confirmation amplicon


Confirmation sequences for line 404F04 and hit At4g13610 are shown.
Line ID 404F04
Locus At4g13610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7920327
Primers used for PCR IN46 - 8474, show primer details
   
Sequence Name 24-K033705-022-404-F04C-8409
GenBank Accession LN496899 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K033705-022-404-F04C-8409
ATTCGGACTGAGCCTTTACATTGAATATATTCATCCGGGAAAGGTGCACCAAATTTAGTA
CTTTGAACAGCCGCATAATGCAAACCTTGAGATAGTGGGATTTTAAAACCCGGATTATTA
AAAACATGCATGGGCTCTGGCCATTCCGGAAGAACTTCGTTTGGTGCAGCAGCCCAAATG
AAAGCTCTTTTACGAGGTTGAGATATTCCATATGCTCCTGCCTCCAAGAGTCCAAATCTC
ACCTACATGTTTCAACAAACTTGTTATATGAAAACCATTGCTTTATATCATATGTAGCCA
ACTTCTACTAAAGAGCAATCATGAATAAGAACGACCATGACACAAAAATAATAGAATTAG
GAAATAAATATTACCTGGTAACCCATTTCAAGAAGAGAAGCTACAGTAAGATGAAATGTA
TGTCCTTCATTGAATGACACAAAGGTTTTCACGTTCTCTAGAAGAAAATACTTTGGCCGG
AAATAGTCAGCAAAAGACAAGAATGCTAATATCATTTGACACTGGTTTTTACTCCAAGAG
CCGTCACTGAACCCTATTT
   
Sequence Name 24-K033705-022-404-F04C-IN46
GenBank Accession LN496900 [GenBank]
Sequencing primer gene specific primer, IN46: 5'-TAAATAGGTTCAGTGACGGCTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K033705-022-404-F04C-IN46
CATTCTTGTCTTTTGCTGACTATTTCCGGCCAAGTATTTTCTTCTAGAGAACGTGAAAAC
CTTTGTGTCATTCAATGAAGGACATACATTTCATCTTACTGTAGCTTCTCTTCTTGAAAT
GGGTTACCAGGTAATATTTATTTCCTAATTCTATTATTTTTGTGTCATGGTCGTTCTTAT
TCATGATTGCTCTTTAGTAGAAGTTGGCTACATATGATATAAAGCAATGGTTTTCATATA
ACAAGTTTGTTGAAACATGTAGGTGAGATTTGGACTCTTGGAGGCAGGAGCATATGGAAT
ATCTCAACCTCGTAAAAGAGCTTTCATTTGGGCTGCTGCACCAAACGAAGTTCTTCCGGA
ATGGCCAGAGCCCATGCATGTTTTTAATAATCCGGGTTTTAAAATCCCACTATCTCAAGG
TTTGCATTATGCGGCTGTTCAAAGTACTAAATTTGGTGCACCTTTCCCGGATGAATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCG
TATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTG
TCTACATTC

Last Updated on 06.12.2018 16:11