Sequences from confirmation amplicon


Confirmation sequences for line 405E05 and hit AT1G01040 are shown.
Line ID 405E05
Locus AT1G01040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28552
Primers used for PCR AY37 - 8474, show primer details
   
Sequence Name 11-K020887-022-405-E05C-8409
GenBank Accession LN490916 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K020887-022-405-E05C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCAACACCACAGGTGACTTGA
GGCCATGTAAGTCCTCGTGATATTAGAAAAGTATTAGTATCTAATGAAACTTAGAAAAAG
GATTGAAGAAATCAAAAAATTATTTGAAGATGTTTTCTTACTTTTGCTTGTAATAGTCAG
CGTACGTGTTGTACTCTAGGGGACCAAGATATCCCTCTTTCCTAGGGAAAGATGTTTCTG
CACTCATGTCATAACAAATTGAATCTACGTAAAACCGCTTCCCGGAATGTGCGGCTGTCA
CTATTTTCCCAATAAGATCTTCTGCAACCATGCACCCATCAGCCATCATCAATTTTCCTT
TTGATAAATCCTCTTCTACTTCCTTCTCAAAAGCATCTCT
   
Sequence Name 12-K020887-022-405-E05C-AY37
GenBank Accession LN491376 [GenBank]
Sequencing primer gene specific primer, AY37: 5'-TTACCTGTGAGAGATGCTTTTGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K020887-022-405-E05C-AY37
AGGAAGTAGAAGAGGATTTATCAAAAGGAAAATTGATGATGGCTGATGGGTGCATGGTTG
CAGAAGATCTTATTGGGAAAATAGTGACAGCCGCACATTCCGGGAAGCGGTTTTACGTAG
ATTCAATTTGTTATGACATGAGTGCAGAAACATCTTTCCCTAGGAAAGAGGGATATCTTG
GTCCCCTAGAGTACAACACGTACGCTGACTATTACAAGCAAAAGTAAGAAAACATCTTCA
AATAATTTTTTGATTTCTTCAATCCTTTTTCTAAGTTTCATTAGATACTAATACTTTTCT
AATATCACGAGGACTTACATGGCCTCAAGTCACCTGTGGTGTTGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
ATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGG
CGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11