Sequences from confirmation amplicon


Confirmation sequences for line 405G07 and hit At5g48160 are shown.
Line ID 405G07
Locus At5g48160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19528338
Primers used for PCR 32tg - 8474, show primer details
   
Sequence Name 33-K116770-0022-405-G07C-32tg
GenBank Accession LN501149 [GenBank]
Sequencing primer gene specific primer, 32tg: 5'-TGGAGAAGGTGGACGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K116770-0022-405-G07C-32tg
AGAGACTTTGAGGAAATGGAGATAGTGGCTGAGGAGAAGATGAGAATGTTCAAGAAGGCA
CGCATGGCTCTTGAGACATGCGATAGAGAGCTCGAAGACAAAGCCAAGGAAGTTTCAGAA
CTGAAAGCAGAGAGGCAGAAGAAGAAACTTCAGATAGATGAGCTAGAGAGAATCGTGAGG
CTGAAGCAAGCGGAGGCAGACATGTTCCAGCTTAAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCAATTATAATA
   
Sequence Name 09-K116769-0022-405-G07C-8409
GenBank Accession LN490360 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K116769-0022-405-G07C-8409
ATTTTAGCTGGACATGTCTGCCTCCGCTTGCTTCAGCCTCACGATTCTCTCTAGCTCATC
TATCTGAAGTTTCTTCTTCTGCCTCTCTGCTTTCAGTTCTGAAACTTCCTTGGCTTTGTC
TTCGAGCTCTCTATCGCATGTCTCAAGAGCCATGCGTGCCTTCTTGAACATTCTCATCTT
CTCCTCAGCCACTATCTCCATTTTCCTCAAAGTCTCTTGTACAACTTCAGCAATTCGGTT
GCATGCATCCTGAGGTGCCATCAAACGTCCACCTTCTCCA

Last Updated on 06.12.2018 16:11