Sequences from confirmation amplicon


Confirmation sequences for line 407B04 and hit At5g66020 are shown.
Line ID 407B04
Locus At5g66020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26402518
Primers used for PCR 06pa - 8474, show primer details
   
Sequence Name 20-K115021-0022-407-B04C-06pa
GenBank Accession LN495334 [GenBank]
Sequencing primer gene specific primer, 06pa: 5'-TTTGTTCTTGACCCTCAATTTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K115021-0022-407-B04C-06pa
TTCTTCTGTGATCAGCATGGAGGTGAAGGGCGCCTAAGTGAGAGGTTTGCAGGTGCTATG
CAGCACATCACTGGTGATGATGTAAGGTATGGCTATGCCTTCTCCTAACATACTTCCTTA
CTACTTGAAGGATATTATTATCAGCTGACTCACTCACTCACTAACTCATCCTCTAGATAC
CTGCACTTTGATTTCCATCACATCTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 07-K115020-0022-407-B04C-8409
GenBank Accession LN489396 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K115020-0022-407-B04C-8409
CATTTACAGATGTGATGGAATCAAAGTGCAGGTATCTAGAGGATGAGTTAGTGAGTGAGT
GAGTCAGCTGATAATAATATCCTTCAAGTAGTAAGGAAGTATGTTAGGAGAAGGCATAGC
CATACCTTACATCATCACCAGTGATGTGCTGCATAGCACCTGCAAACCTCTCACTTAGGC
GCCCTTCACCTCCATGCTGATCACAGAAGAAGTAATTTACTTTTTGGACTATATGATTTA
ATACAAAATTGAGGGTCAAGAACAA

Last Updated on 06.12.2018 16:11