Sequences from confirmation amplicon


Confirmation sequences for line 412E11 and hit At1g44350 are shown.
Line ID 412E11
Locus At1g44350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:16836237
Primers used for PCR 20lg - 8474, show primer details
   
Sequence Name 40-K113947-0022-412-E11C-20lg
GenBank Accession LN503780 [GenBank]
Sequencing primer gene specific primer, 20lg: 5'-CTAATTTTGGACTGGTTTGGTCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K113947-0022-412-E11C-20lg
GTTTTGGCCCGCTTTTTATAATAGCAATAAACAAAAATTCCTAATCTAAGTGGTTATGGT
CTATGGTACGCACCTCTTGAATACGTTTCTTGAGGTAGTAGAAGCTTGAGTTAGAAAAAG
CTCTGAAAGTACCACCGAGGACCACCGTGTCCGGCGCCACATCGAGACTATGACCGCCAT
CAAATGAAGTAACCGACACAACCTACAACTCGAGAAAATGGGAATCAGAATTAAGCTATA
AATTTTCAAATATCTGGTTGTGGATTTTATGAAGTACCTGAGAGTCAAGAGGACTAGCTT
CACGGGATACAATGCCTTGAAGACTAATGACCGCGGAAGAAGCTGCAAGAAGGAGATTAG
CGGCGCCACGGCTATCTTCCGAAGTGATAACCGCCCGGAAAATTCCACATCCCGCGAGCA
AAGGACCACTTCTTGATCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 12-K113946-0022-412-E11C-8409
GenBank Accession LN491664 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K113946-0022-412-E11C-8409
ATTTACATTGAAATATCCTGGATCAAGAAGTGGTCCTTTGCTCGCGGGATGTGGAATTTT
CCGGGCGGTTATCACTTCGGAAGATAGCCGTGGCGCCGCTAATCTCCTTCTTGCAGCTTC
TTCCGCGGTCATTAGTCTTCAAGGCATTGTATCCCGTGAAGCTAGTCCTCTTGACTCTCA
GGTACTTCATAAAATCCACAACCAGATATTTGAAAATTTATAGCTTAATTCTGATTCCCA
TTTTCTCGAGTTGTAGGTTGTGTCGGTTACTTCATTTGATGGCGGTCATAGTCTCGATGT
GGCGCCGGACACGGTGGTCCTCGGTGGTACTTTCAGAGCTTTTTCTAACTCAAGCTTCTA
CTACCTCAAGAAACGTATTCAAGAGGTGCGTACCATAGACCATAACCACTTAGATTAGGA
ATTTTTGTTTATTGCTATTTATTAAAAAGCGGGCCAAAACCAATGATTAAAAGGTTCCAA
CTATAATTCTCACATGACCAAACCANGTCAAAATTA

Last Updated on 06.12.2018 16:11