Sequences from confirmation amplicon


Confirmation sequences for line 412F04 and hit At5g36870 are shown.
Line ID 412F04
Locus At5g36870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14532799
Primers used for PCR VL43 - 8474, show primer details
   
Sequence Name 09-K024206-022-412-F04C-VL43
GenBank Accession LN490006 [GenBank]
Sequencing primer gene specific primer, VL43: 5'-ATGACGCCCTATTTGATTTTGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K024206-022-412-F04C-VL43
TGATGCAGCTACAACTTGCTTCCGTATTTTTCACATTTCAACTTGGGACAAAATTTCATT
ACTACTGCAAGACATTGTTGCATGGAGGCGCAGAATACAGAGGTACTGGCCGTGGATTTG
TGGTGTTCCATGCCAAATTTGCTGAAAACTACAGGTTTTACTCTCGTAGTCATTTTGTGA
AGGCTACTGAGCTAGGGATTCTCCTACTCGTGTATCACATATTTGGCCCAACCTATATAG
GGTTGTTCACTATTTCGATATGGTTCATGGTGGGTACATGGCTCTTTGCTCCTTTCTTAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCG
TATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTG
TCTACATTCA
   
Sequence Name 10-K024206-022-412-F04C-8409
GenBank Accession LN490479 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K024206-022-412-F04C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATAAGAAAGGAGCAAAGAGCCATGTA
CCCACCATGAACCATATCGAAATAGTGAACAACCCTATATAGGTTGGGCCAAATATGTGA
TACACGAGTAGGAGAATCCCTAGCTCAGTAGCCTTCACAAAATGACTACGAGAGTAAAAC
CTGTAGTTTTCAGCAAATTTGGCATGGAACACCACAAATCCACGGCCAGTACCTCTGTAT
TCTGCGCCTCCATGCAACAATGTCTTGCAGTAGTAATGAAATTTTGTCCCAAGTTGAAAT
GTGAAAAATACGGAAGCAAGTTGTAGCTGCATCAATACAAAATCAAATAGGGCGTCAT

Last Updated on 06.12.2018 16:11