Sequences from confirmation amplicon


Confirmation sequences for line 434C06 and hit At4g01750 are shown.
Line ID 434C06
Locus At4g01750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:756520
Primers used for PCR 51yz - 8474, show primer details
   
Sequence Name 54-K123255-0022-434-C06C-51yz
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 51yz: 5'-AAGTTGAAGAAACCCTAGTCGGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K123255-0022-434-C06C-51yz
TATGTTTTCTTACTAGAAACAGGGGATACATAATCATTTGGATTTGATCAAGATCCGAAA
GAAAAATGACTGCTACTACATCCACAGGTTGACCATCTTTTGTTATTTTATTAGAGGATC
AACCATTTGGGAATAATGTCCCACATCGGAAACTGGGCAAAGATCTTAAAGTGTACCACA
TAGAAAAGGATATGGACCAATCCATTCATTGTCAATTGGTTTTGTGTTGGAACCTCATAA
TTTAATACTAAACACTTCTCAAAACAAAAATCTTATAGACTACTCAATTTTATGATCCCA
GTAATCACTCAGAGCTAATCAAGAACATCCAAGATCTAAGTATGATGAAGGCGTGGTTAA
GGAATCCCATGACCAAAATGTAATGGTATCTATAGTAATATATGCAAGTCACATTAGCCA
ATCTACTCATTGCGAGTATATAAGCCATTTCCAGCTAGATCTCAGTAACTGAAAGCTAAA
GATATCTCAAAGGGGAAAAGTTTACCTGGGAACCGAAACTGTATGCGGTTTTAGAATCGA
GCGCTGGAGGAATAAGAACGGCGTGGCCAGGCCACTTTTCGTTGACTTTATACAGAGTAA
TGTAATCCTCAGCGATCACAAGGACTTTTTCTTGATGCTTCTGTCTAGAAACACTAATCA
ACCAGTTGTTGAGAAAAGGCAAGAATGGTGAGCTCACGGCGCAGACGATCACAGTGCCGT
TCGTGGCGACAAACTTAGCTGCCTGAGCAAGGGTATAGTTACGCCATTCAGATTTGGCAT
GCGGAGACAAAGAAGAAGCATAAGAAGGAGAAGAGACTTTGTTCGGAAATAGCAACAAAG
GAGATCCGGGCCAAGGTAAGAATACACCGAGGAGAANGAAGAGAGCGAGAAGGACAAGGA
GGAGACCGTTGCGGCTA
   
Sequence Name 22-K123254-0022-434-C06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K123254-0022-434-C06C-8409
ATTTCATTGAATATATCCTGTTGCTACAACAATGGCGCAGAAACAACAGACCCTTCACCA
ACAACGACCATTCTCTTCTTCTCCTCGTTCTTATTCTTCAATCTCAAACCGTCCCATCTT
TCTCCTTAGCCGCAACGGTCTCCTCCTTGTCCTTCTCGCTCTCTTCCTTCTCCTCGGTGT
ATTCTTACCTTGGCCCGGATCTCCTTTGTTGCTATTTCCGAACAAAGTCTCTTCTCCTTC
TTATGCTTCTTCTTTGTCTCCGCATGCCAAATCTGAATGGCGTAACTATACCCTTGCTCA
GGCAGCTAAGTTTGTCGCCACGAACGGCACTGTGATCGTCTGCGCCGTGAGCTCACCATT
CTTGCCTTTTCTCAACAACTGGTTGATTAGTGTTTCTAGACAGAAGCATCAAGAAAAAGT
CCTTGTGATCGCTGAGGATTACATTACTCTGTATAAAGTCAACGAAAAGTGGCCTGGCCA
CGCCGTTCTTATTCCTCCAGCGCTCGATTCTAAAACCGCATACAGTTTCGGTTCCCAGGT
AAACTTTTCCCCTTTGAGATATCTTTAGCTTTCAGTTACTGAGATCTAGCTGGAAATGGC
TTATATACTCGCAATGAGTAGATTGGCTAATGTGACTTGCATATATTACTATAGATACCA
TTACATTTTGGTCATGGGATTCCTTAACCACGCCTTCATCATACTTAGATCTTGGATGTT
CTTGATTAGCTCTGAGTGATTACTGGGATCATAAAATTGAGTAGTCTATAAGATTTTTGT
TTTGAGAAGTGTTTAGTATTAAATTATGAGGTTCCAACACAAAACCAATTGACAATGAAT
GGATTGGTCCATATCCTTTTCTATGTGGTACACTTTAAGATCTTTGCCCAGTTTCCGATG
TGGGACATTATTCCCAAATGGTTGATCCTCTAATAAAATAACAAAAGATGGTCAACCTGT
GAATGTAGTAGCAGTCATTTTTCTTTCGGATCTTGATCAAATCCAAAT

Last Updated on 06.12.2018 16:11