Sequences from confirmation amplicon


Confirmation sequences for line 439E10 and hit At4g03415 are shown.
Line ID 439E10
Locus At4g03415 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1505221
Primers used for PCR JV39 - 8474, show primer details
   
Sequence Name 10-K101324-0022-439-E10C-8409
GenBank Accession LN490583 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K101324-0022-439-E10C-8409
CATTTACATTGAATATATAGTGGGTATTTCTAACACTATGGTCAACTATAAAGCCGGATT
TCTTTGTTGCTTTGTAGGATTTTATGTCTGAAGATGTGACATTTTGTGGTGTATTTGATG
GTCATGGTCCTTATGGCCATCTTGTTGCTCGTAAAGTGAGAGATACATTGCCTGTGAAGT
TACAGTTTTTCTTTCAGACGCTTCAGTCGAAGCAAAACTGTTCTAAAGGAACTCGATTCA
GAAGAAATTCAAGCAAATCAGCTGTCCAAGAAGCTGTTAAAGAAGGATCTGACGAGGATA
AACTTAAAGGCTTATGGGGAGAAGCTTTCTTGAAATCTTTTAAAGCCATGGATAAGGAAC
TGCGGTCTCATCCTAATCTGGACTGTTTTTGTAGTGGCAGCACTGGCGTAACAATTCTTA
AACAGGTACACTTAGAGAACAAAGAGCTCTCTTTCAGCTTCAACCATGTTTTCTTTAATC
ATCTCTNTTGATTGTTCAGGGNTCTAAT
   
Sequence Name 10-K101341-0022-439-E10C-JV39
GenBank Accession LN490584 [GenBank]
Sequencing primer gene specific primer, JV39: 5'-GATTAGACCCCTGAACAATCAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K101341-0022-439-E10C-JV39
CTGAAGAGAGCTCTTTGTTCTCTAGTGTACCTGTTTAAGAATTGTTACGCCAGTGCTGCC
ACTACAAAAACAGTCCAGATTAGGATGAGACCGCAGTTCCTTATCCATGGCTTTAAAAGA
TTTCAAGAAAGCTTCTCCCCATAAGCCTTTAAGTTTATCCTCGTCAGATCCTTCTTTAAC
AGCTTCTTGGACAGCTGATTTGCTTGAATTTCTTCTGAATCGAGTTCCTTTAGAACAGTT
TTGCTTCGACTGAAGCGTCTGAAAGAAAAACTGTAACTTCACAGGCAATGTATCTCTCAC
TTTACGAGCAACAAGATGGCCATAAGGACCATGACCATCAAATACACCACAAAATGTCAC
ATCTTCAGACATAAAATCCTACAAAGCAACAAAGAAATCCGGCTTTATAGTTGACCATAG
TGTTAGAAATACCCACTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11