Sequences from confirmation amplicon


Confirmation sequences for line 450E06 and hit At4g19600 are shown.
Line ID 450E06
Locus At4g19600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10673736
Primers used for PCR 22us - 8474, show primer details
   
Sequence Name 48-K117387-0022-450-E06C-22us
GenBank Accession LN506624 [GenBank]
Sequencing primer gene specific primer, 22us: 5'-TAAAGGACTATTGGGCCATACAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K117387-0022-450-E06C-22us
TTTTGTGCCATAAGGCATAACTCTGACTAGCTTATTCATGCTCTAATGACCTCTACGATA
TCAAGTTCTATAAATTTCAAAATTCGATCCTACAGAACCTAATGTAAGGGGATCAAGAAT
TCACTATAATACTATAAGCAAAATAGAGGCAATTATCATGGTAGGAGAAACTAGCATGTC
CTAGACCAAGCTCGCAAGAAGTAATCAAATGGAAAATGCAATGTATAAAGCTGAAATATA
TAGGTCAGAAATTTATACTTACACTTTTAATCTCATGCCTAAATCCTGCAAAAATGTACA
GTATGACTTGCGGAGGTATGTTTCCTTCTTTAAGTCAATACTATCCAACCTTGAAGGTGA
ATTTTCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCG
   
Sequence Name 21-K117386-0022-450-E06C-8409
GenBank Accession LN495835 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K117386-0022-450-E06C-8409
ATTTACATTGAAGAAATTCACCTTCAAGGTTGGATAGTATTGACTTAAAGAAGGAAACAT
ACCTCCGCAAGTCATACTGTACATTTTTGCAGGATTTAGGCATGAGATTAAAAGTGTAAG
TATAAATTTCTGACCTATATATTTCAGCTTTATACATTGCATTTTCCATTTGATTACTTC
TTGCGAGCTTGGTCTAGGACATGCTAGTTTCTCCTACCATGATAATTGCCTCTATTTTGC
TTATAGTATTATAGTGAATTCTTGATCCCCTTACATTAGGTTCTGTAGGATCGAATTTTG
AAATTTATAGAACTTGATATCGTAGAGGTCATTAGAGCATGAATAAGCTAGTCAGAGTTA
TTGCCTTATTGGCAACAAAAAGGTGAAACTTGTGGGTTCATCTTGTGTTGTATGGCCCAT
AGGTCCTTT

Last Updated on 06.12.2018 16:11