Sequences from confirmation amplicon


Confirmation sequences for line 452H11 and hit At1g09340 are shown.
Line ID 452H11
Locus At1g09340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3017561
Primers used for PCR MN12 - 8474, show primer details
   
Sequence Name 27-K028159-022-452-H11C-8409
GenBank Accession LN498242 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K028159-022-452-H11C-8409
ATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATCCAAATCGTTTCAAGTACATCC
TGGTGTGGTCCGTTTGTAATAGATATCATACTCTGAGCTTTTTGGATCATTGCTTGAGTA
AACTTATTCATGTTTTCAACTCTCTCTCAGGCCGGTGGGTTTCCGGAGCCAGAGATTGTT
CATTACAACCCGAAAGAGTTCGACTTTGGGAAGAAGAAGGCATTCCCTTTCCGTGATCAG
GTAAAAACCACAACGTTCTAATGATCGAGGCTGCAACATGAACGATTCCAATTTAGAAGT
TGAGATTTTGATATATGTATATTCTCTTGCAGCATTTCTTTGCATCGGTGGAGAAAGCAA
AGCATGTCCTCGGATGGAAACCGGAGTTCGACTTAGTGGAGGGTCTCACTGACTCATACA
ACCTTGATTTCGGTCGCGGAACATTCCGGAAAGAAGCGGATTTCACCACTGACGACATGA
TTCTGAGCAAGAAACTTGTTCTTCAATAATCGAAATCCTAAGAGTTGCTCATTCTTGGCT
TGTATGATTCTGATCACCCGGTTCTCTTAAAGTTCTGAACTTTATTGTCATCTCACGTAT
GTTATGGTCCGGATTTTGTTCGACTTTTCTCTAAAGAAGTCAAGCTAGGGACGATG
   
Sequence Name 28-K028159-022-452-H11C-MN12
GenBank Accession LN498705 [GenBank]
Sequencing primer gene specific primer, MN12: 5'-TTACTTTCTCGGTTTCTTCATCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K028159-022-452-H11C-MN12
CCCTAGCTTGACTTCTTTAGAGAAAAGTCGAACAAAATCCGGACCATAACATACGTGAGA
TGACAATAAAGTTCAGAACTTTAAGAGAACCGGGTGATCAGAATCATACAAGCCAAGAAT
GAGCAACTCTTAGGATTTCGATTATTGAAGAACAAGTTTCTTGCTCAGAATCATGTCGTC
AGTGGTGAAATCCGCTTCTTTCCGGAATGTTCCGCGACCGAAATCAAGGTTGTATGAGTC
AGTGAGACCCTCCACTAAGTCGAACTCCGGTTTCCATCCGAGGACATGCTTTGCTTTCTC
CACCGATGCAAAGAAATGCTGCAAGAGAATATACATATATCAAAATCTCAACTTCTAAAT
TGGAATCGTTCATGTTGCAGCCTCGATCATTAGAACGTTGTGGTTTTTACCTGATCACGG
AAAGGGAATGCCTTCTTCTTCCCAAAGTCGAACTCTTTCGGGTTGTAATGAACAATCTCT
GGCTCCGGAAACCCACCGGCCTGAGAGAGAGTTGAAAACATGAATAAGTTTACTCAAGCA
ATGATCCAAAAAGCTCAGAGTATGATATCTATTACAAACGGACCACACCAGGATGTACTT
GAAACGATTTGGATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT

Last Updated on 06.12.2018 16:11