Sequences from confirmation amplicon


Confirmation sequences for line 456E05 and hit At5g27750 are shown.
Line ID 456E05
Locus At5g27750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9829078
Primers used for PCR 34yo - 8474, show primer details
   
Sequence Name 15-K121121-0022-456-E05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K121121-0022-456-E05C-8409
CGTCCGAGATCTCACACGAGTAACCGGCATTTCATCGTCATCAATATGACATATCAAGGT
CCTTACCAAGGTCAGGTCTTCAAGAACAGGACATCCAGAGACAAGCTTCTCAAGACTCAT
GGTACCAGACCATCGAACTTCACGAAGAATCATGAACTTAAGACAAGGCATAGAAAGCAA
ACCCGGATCGTTAAGAGCCGAATCCCGGAGCTTTAAAGTAACCAACGTCTTGCTTGTAAA
GAGATTCAGAGGCATGAACTCCAAATAAGGATACATAATGCATTCCTCCTCCCAATACAT
GGTACTACTCACAGCATCTAGAAGTCGAATTCCGCGGTTAATCGCTGTACCGATCCGATC
CTTGAACCCGCGTATCTCACGCCTTTTGTAATCTACCTCGAATGTTTGCAGGCGTGACTC
AGGATTAAACTCTAGAAACCTGTCGATGAAGTTAATGAACACTTGTTCATATTCTTCATC
TTCAAAAGGGAAATCACTGCAAGTTAAGTCAAGTCCGGGAACATTTAGCCATAGATTCTT
CCACCTAGTCGATAAAACACTCGTCTTCACCGAATCTTTAGTCGGAAGGCATAAGAGTAT
TTGAGTAATCAGAGATTCCGGTAATTCGCTAATTCTATCGAACCCAGGCATCACGAGACA
CAAAACCCTAAGAGCATGAACATACAACAACAATGTAACTTGATTAAATCCTAGGCGTTG
A
   
Sequence Name 31-K121122-0022-456-E05C-34yo
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 34yo: 5'-TTAATCGATTCATAACTCAACGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K121122-0022-456-E05C-34yo
TTCTGCTCTTAGGGTTTTGTGTCTCGTGATGCCTGGGTTCGATAGAATTAGCGAATTACC
GGAATCTCTGATTACTCAAATACTCTTATGCCTTCCGACTAAAGATTCGGTGAAGACGAG
TGTTTTATCGACTAGGTGGAAGAATCTATGGCTAAATGTTCCCGGACTTGACTTAACTTG
CAGTGATTTCCCTTTTGAAGATGAAGAATATGAACAAGTGTTCATTAACTTCATCGACAG
GTTTCTAGAGTTTAATCCTGAGTCACGCCTGCAAACATTCGAGGTAGATTACAAAAGGCG
TGAGATACGCGGGTTCAAGGATCGGATCGGTACAGCGATTAACCGCGGAATTCGACTTCT
AGATGCTGTGAGTAGTACCATGTATTGGGAGGAGGAATGCATTATGTATCCTTATTTGGA
GTTCATGCCTCTGAATCTCTTTACAAGCAAGACGTTGGTTACTTTAAAGCTCCGGGATTC
GGCTCTTAACGATCCGGGTTTGCTTTCTATGCCTTGTCTTAAGTTCATGATTCTTCGTGA
AGTTCGATGGTCTGGTACCATGAGTCTTGAGAAGCTTGTCTCTGGATGTCCTGTTCTTGA
AGACCTGACCTTGGTAAGGACCTTGATATGTCATATTGATGACGATGAATTGCCGGTTAC
TCGTGTGAGATCTCGGACGTCTTCAGACTCCAGACAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTNCATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTATCAATCAAAAATGTAGATGT

Last Updated on 06.12.2018 16:11