Sequences from confirmation amplicon


Confirmation sequences for line 461B08 and hit At1g18180 are shown.
Line ID 461B08
Locus At1g18180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6257670
Primers used for PCR 25ny - 8474, show primer details
   
Sequence Name 40-K114669-0022-461-B08C-25ny
GenBank Accession LN503799 [GenBank]
Sequencing primer gene specific primer, 25ny: 5'-CAAAGTTTAAACCAGCTTTCCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K114669-0022-461-B08C-25ny
CGGGAGGCTTGTGATGCAACTAAACGCTATATTCATCTCGGGAACCGTCTTCTTGCGTAG
AAACCCGACTGCCAAGATATGGGCTGTGGTCTATCTGGTTTGCCTTCATCTATGGGTATT
ATACATCCTACTCTCTCATTCCGATGCTTCTTCTTCAGGTGAACTTAGATCAGGTGCAGT
GATTTCCTTAGAGAACTTCAGTAACAGTTCACTTCAATAGCCTTTTCCTTAGAGAATGTT
TCTTTTGATTCATATGTAATTTTCCTTCCAACATTTTTTTTGAAGCTTGGTTTGAATCTN
ATTGTATTCTCAATCAATGTAGCAATTATCTTTAACAAGTACCCAAAGACAAACAAGAAT
CTATACAAAAAGAGAGTTTGAATATTAAGGAAGAACTAGGAGACGGGTTTGCAAAATCAG
TGACAATCTACTCTCTTTCTCTCTCTCAGTCAATGCTTCTCTGGCTCTTCTTCGAGTTTC
GTCTATCCCTTGCACGTAGAAATAACTCANAAGTTATCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 17-K114668-0022-461-B08C-8409
GenBank Accession LN493959 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K114668-0022-461-B08C-8409
CTTATGAGTTATTTCTACGTGCAAGGGATAGACGAAACTCGAAGAAGAGCCAGAGAAGCA
TTGACTGAGAGAGAGAAAGAGAGTAGATTGTCACTGATTTTGCAAACCCGTCTCCTAGTT
CTTCCTTAATATTCAAACTCTCTTTTTGTATAGATTCTTGTTTGTCTTTGGGTACTTGTT
AAAGATAATTGCTACATTGATTGAGAATACAATAAGATTCAAACCAAGCTTCAAAAAAAA
TGTTGGAAGGAAAATTACATATGAATCAAAAGAAACATTCTCTAAGGAAAAGGCTATTGA
AGTGAACTGTTACTGAAGTTCTCTAAGGAAATCACTGCACCTGATCTAAGTTCACCTGAA
GAAGAAGCATCGGAATGAGAGAGTAGGATGTATAATACCCATAGATGAAGGCAAACCAGA
TAGACCACAGCCCATATCTGGGCAGTCGGGTTTCTACGCAAGAAGACGGTTCCCGAGATG
AATATAGCGTTTAGTTGCATCACAAGCCATCCCAAATGTTTCTTGCCTGACCGGATTTTA
TCTTGGAA

Last Updated on 06.12.2018 16:11