Sequences from confirmation amplicon


Confirmation sequences for line 472F11 and hit At5g15230 are shown.
Line ID 472F11
Locus At5g15230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4945409
Primers used for PCR DB20 - 8474, show primer details
   
Sequence Name 30-K029911-022-472-F11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K029911-022-472-F11C-8409
ACTGTCCATTGACACAATCATTTCGTGGATTTGCGAT
   
Sequence Name 30-K029911-022-472-F11C-DB20
GenBank Accession LN499596 [GenBank]
Sequencing primer gene specific primer, DB20: 5'-TATAAAAAGGGAACGAAGGGAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K029911-022-472-F11C-DB20
TTTTCAGTTGTTGTAGCAGGAGCAAANCTTGTTTGTTCCCATAGTAACCCNGGAGGCACA
CAGAGACACTTCCTGCAGCATTTGTTGCAGAACGTAATGCAAGCCTTGTGGTACTGTGTC
TTTTTACACCTCCTATCACATTCCGATGGGCATTCTGGTTGTTTCAAATACAAACAATAT
CAAAATGTTAACACTCAAAAATTTTTTATTACTAATAAAAACAACAGAATCTAGTGGAAA
TTCATTAGCTTTTTTTTTTACTGTTTTGTGGGTCTCAAGATAGTGGGTTGCTTTGACTGT
ATGACCATATTAAGGGGCAATTTCGTCAATATCTAATATATTTTGAGTCTACGGATCTGA
CCAAACAGTCCACTTAACACTTCACTTTCAACTTATTTTCTGCAACTGAAATGCTTATTT
TTTTTTATTATAACACAATAAAAGATTTCTTGAATTGTTTATTTTTCGTCGATTATGAGA
TTAAAAAAAAATTGTTTAAAAATAGCTGTGAAAAAAAAACTTACGGGTACGTTTCAGGCT
TCCTGGTCCATAACGTTTCTGAAAATTAACCAAAAAAGGTAACTAATTATATACCAGCAA
TGAGTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAAATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATT

Last Updated on 06.12.2018 16:11