Sequences from confirmation amplicon


Confirmation sequences for line 483G04 and hit At5g20000 are shown.
Line ID 483G04
Locus At5g20000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6757560
Primers used for PCR MC13 - 8474, show primer details
   
Sequence Name 80-K035350-022-483-G04C-MC13
GenBank Accession LN513905 [GenBank]
Sequencing primer gene specific primer, MC13: 5'-ACCAATCATGTCATATGTGGAGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K035350-022-483-G04C-MC13
TAGGTTACCAAGGGATCTACTTTACTTGGCAGAACCAGGTGGAGAACATAGCTATCATTA
CGGAGAGCAACTCTCGTTGATGGAGTGAGTTTCGTTATGTCTATACTTTTGTCAATATCG
ACAACATACTTCCCCTCTGGATGAACCTGAAACATGGGAAAGTATTATTCTATCAATGTT
CATATGATTGCAAACAATTCAAAAACCAATCTAGATAATACGATAAAAACATTCATACCT
TAACCAAGACCTTGTTTTTTCCCATCACTTTTACCAAAAATCGACATGGCTCCTTGTCCA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTC
GTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGT
GTCTACATTCACTCCCAAAATGG
   
Sequence Name 32-K035350-022-483-G04C-8409
GenBank Accession LN500488 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K035350-022-483-G04C-8409
GACAAGGAGCCATGTCGATCTTTGGTAAAAGTGATGGGAAAAAACAAGGTCTTGGTTAAG
GTATGAATGTTTTTATCGTATTATCTAGATTGGTTTTTGAATTGTTTGCAATCATATGAA
CATTGATAGAATAATACTTTCCCATGTTTCAGGTTCATCCAGAGGGGAAGTATGTTGTCG
ATATTGACAAAAGTATAGACATAACGAAACTCACTCCATCAACGAGAGTTGCTCTCCGTA
ATGATAGCTATGTTCTCCACCTGGTTCTGCCAAGTAAAGTAGATCCCTTGGTTAACCTTA
TGAAAGTTGAGAAGGTTCCAGACTCCACATATACATGGATTGG

Last Updated on 06.12.2018 16:11