Sequences from confirmation amplicon


Confirmation sequences for line 534A11 and hit At1g77330 are shown.
Line ID 534A11
Locus At1g77330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29064320
Primers used for PCR 07gt - 8474, show primer details
   
Sequence Name 46-K112250-0022-534-A11C-07gt
GenBank Accession LN505905 [GenBank]
Sequencing primer gene specific primer, 07gt: 5'-GAGATTTTTGAAAATGGGTTTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K112250-0022-534-A11C-07gt
ATGTTACTTAATGTTGGATGGCCATTCGTTTTGGTTATGGTCCAAGAGAGTGAAGACATC
TTCCCAGTCCACGTTTTCTAGCTTCTCGCCAGAGTTCTTTTGAACCAATTCGTTGAGCAA
CTTCACGGGATTAGAAGTCTTGAATGCTTCTTCTCTCTCTGTTTTGTAGCAATCTGAGCT
CAGCTTCTTCACCTTATTTAGAAGCTCCAATGGAATACCATGGTTCACCAGCTACATACA
TACACAGATAAGCACATGAAAACAGAGAGACAGAGAGAGTGTGTGTTCATCAATCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 36-K112249-0022-534-A11C-8409
GenBank Accession LN502277 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K112249-0022-534-A11C-8409
TCATTGATTGATGAACACACACTCTCTCTGTCTCTCTGTTTTCATGTGCTTATCTGTGTA
TGTATGTAGCTGGTGAACCATGGTATTCCATTGGAGCTTCTAAATAAGGTGAAGAAGCTG
AGCTCAGATTGCTACAAAACAGAGAGAGAAGAAGCATTCAAGACTTCTAATCCCGTGAAG
TTGCTCAACGAATTGGTTCAAAAGAACTCTGGCGAGAAGCTAGAAAACGTGGACTGGGAA
GATGTCTTCACTCTCTTGGACCATAACCAAAACGAATGGCCATCCAACATTAAGTAAACA
TTTTCTCTTTTAAAACTAGTCAAAATCTTTCAAACCCATTTCAAAAAAATCT

Last Updated on 06.12.2018 16:11