Sequences from confirmation amplicon


Confirmation sequences for line 535C08 and hit At5g08415 are shown.
Line ID 535C08
Locus At5g08415 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2712629
Primers used for PCR 15rx - 8474, show primer details
   
Sequence Name 35-K116272-0022-535-C08C-15rx
GenBank Accession LN501965 [GenBank]
Sequencing primer gene specific primer, 15rx: 5'-CTCAATTACGAAACCCACTTTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K116272-0022-535-C08C-15rx
GGTTTTCAGAATCCGATGCGAATCCGGGGATGTTTCGTCGCCATTGCGGACCAAGGCGGT
GTCTTTGTCGTCGGAGATGGAGGATTCGTCGTCCTTGAAGAAGAGTTTGATGGAGTTAGA
AGGGAAGAAGAGTGAGCCTTACCCAGGAGGAATGCCGAAGATGGGTCCATTCACAGGGAG
AGATCCGAATGTGAAGAAACCTGCTTGGTTGAGACAGAAGGCACCACAAGGCGAAAGGTT
TCAGGAGGTGAAAGAGTCCCTTTCTCGTCTCAATCTCAATACTGTTTGTGAAGAAGCTCA
GTGTCCTAATATCGGCGAGGTTAGCTATTTCGATTTTGTGTTTATGATCTCGAAGATTGT
TATTAAAATGTATCGGTTTTATGAATTGTGGGTATGAGAAATTATGAGTAAACTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 11-K116271-0022-535-C08C-8409
GenBank Accession LN491276 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K116271-0022-535-C08C-8409
TAAAACCGATACATTTTAATAACAATCTTCGAGATCATAAACACAAAATCGAAATAGCTA
ACCTCGCCGATATTAGGACACTGAGCTTCTTCACAAACAGTATTGAGATTGAGACGAGAA
AGGGACTCTTTCACCTCCTGAAACCTTTCGCCTTGTGGTGCCTTCTGTCTCAACCAAGCA
GGTTTCTTCACATTCGGATCTCTCCCTGTGAATGGACCCATCTTCGGCATTCCTCCTGGG
TAAGGCTCACTCTTCTTCCCTTCTAACTCCATCAAACTCTTCTTCAAGGACGACGAATCC
TCCATCTCCGACGACAAAGACACCGCCTTGGTCCGCAATGGCGACGAAACATCCCCGGAT
TCGCATCGGATTCTGAAACCCAAATTCAAGAATTTACTGGAATGATGATGAAGCTTCTGG
GTCGAGATCGAAATTGAGAAAGTGGGTT

Last Updated on 06.12.2018 16:11