Sequences from confirmation amplicon


Confirmation sequences for line 541F02 and hit At1g53450 are shown.
Line ID 541F02
Locus At1g53450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19953552
Primers used for PCR 22vh - 8474, show primer details
   
Sequence Name 23-K117806-0022-541-F02C-22vh
GenBank Accession LN496736 [GenBank]
Sequencing primer gene specific primer, 22vh: 5'-CTCTCACAGGGGAATTTGAAGTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K117806-0022-541-F02C-22vh
GAATGTTCATACTACTTGTCCTAGTTTCATAACTTGATGATAGCTCAACCGTTCCCTACA
AGGAGCAGAAACTGCAAGCTGTAAACAAATTTCACTCTTGGCAGGAACTCCATTAAACTT
GATTCAAAACATCAATAATTTCTTCCAGACTCTTATTGATTGAAGCCATAGCCTAAAACA
ATCCTATTTTCGCAAGGTAGTAGTAATCAGAAAGCCCCTAAATTCCAAAACTTTGCATAT
GCTCATTCTCAATCTCTAAGAAATCTCCTACATTCAAATACAATCTAGATATCTCTGCAG
TAAGAAACAAAATAAAAACTTGAAATTGCAGAACTACAACAAGTACCTTTGCTCTCCTAT
GTAATCCAATAGTCCTTAAATCGAATTCTGTAACTTTCTCCTCATACACAGTACCAGAGG
AAGCAGTATCTGTCTTAGAAAACGCTGAGTTCTCCTTTGTGTCTACATAAGCCCTATGGC
TCCTCGTGACAGTATCTCTCTCAGTAGAAACAAACACGTTCTCATCGTGTCGGAACGGTA
CAGGCAACCTTCTGAAGAACGGTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 23-K117784-0022-541-F02C-8409
GenBank Accession LN496735 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K117784-0022-541-F02C-8409
ATTTACAATTGAATATATCCTGTACCGTTCTTCGAAGGTTGCCTGTACCGTTCCGACACG
ATGAGAACGTGTTTGTTTCTACTGAGAGAGATACTGTCACGAGGAGCCATAGGGCTTATG
TAGACACAAAGGAGAACTCAGCGTTTTCTAAGACAGATACTGCTTCCTCTGGTACTGTGT
ATGAGGAGAAAGTTACAGAATTCGATTTAAGGACTATTGGATTACATAGGAGAGCAAAGG
TACTTGTTGTAGTTCTGCAATTTCAAGTTTTTATTTTGTTTCTTACTGCAGAGATATCTA
GATTGTATTTGAATGTAGGAGATTTCTTAGAGATTGAGAATGAGCATATGCAAAGTTTTG
GAATTTAGGGGCTTTCTGATTACTACTACCTTGCGAAAATAGGATTGTTTTAGGCTATGG
CTTCAATCAATAAGAGTCTGGAAGAAATTATTGATGTTTTGAATCAAGTTTAATGGAGTT
CCTGCCAAGAGTGAAATTTGTTTACAGCTTGCAGTTTCTGCTCCTTGTAGGGAACGGTTG
AGCTATCATCAAGTTATGAAACTAGGACAAGTAGTATGGAACATTCGTTAGCAGCCAGGG
GAGATCTTTGGAGAGTAGAAGCTTCTACTTCAAATTCCCATGGTGAGAGAAACAAGATTG
AGTAATTACCAATTTTTTTTCACTTCAGAAATGTAAATATCCGCA

Last Updated on 06.12.2018 16:11