Sequences from confirmation amplicon


Confirmation sequences for line 541F11 and hit At1g49730 are shown.
Line ID 541F11
Locus At1g49730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18402741
Primers used for PCR 35lj - 8474, show primer details
   
Sequence Name 41-K113947-0022-541-F11C-35lj
GenBank Accession LN504156 [GenBank]
Sequencing primer gene specific primer, 35lj: 5'-TGAATTTGCAGCTAAAGGAATTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K113947-0022-541-F11C-35lj
TGCTTGAGAAGACCCTTACCGGTCCGTGGTAAGAAGGTGAAGTCAAAGCAAGAAGAACCT
GCTAATGTAATACAGGACCACTCAAAAGGAAGACACTGGGAGAGTAATATGTAATAGAAG
ATAGTGCTACTGTACAGGAGAAATTACAGAGACGCTTACAATGTAGAAATCTTTTGAGCT
GAATTTAACTAAGAGTGCAGTCTGTGTAGAGTATGAGAGCTTTCAATATGAATTCATAAT
TTTCATAAACATATGTAAAACTTTCAGATTTAGCTATAGAGAAGATGTGACTAACTCAGA
AAGAAAATCCGTTAATTGGAGAGTGAGGTAAACTCCGGCTGTAATGTGACCTGGAAGTAG
TACTAGACGATGGACCAAAGATCCGGGAATCACCTCTTTGAATTCTCAAATTGCTTCTCA
ACCTATCCGATATATTCAATTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 13-K113946-0022-541-F11C-8409
GenBank Accession LN492124 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K113946-0022-541-F11C-8409
TCATTGAAATATCCTGCAATTGAATATATCGGATAGGTTGAGAAGCAATTTGAGAATTCA
AAGAGGTGATTCCCGGATCTTTGGTCCATCGTCTAGTACTACTTCCAGGTCACATTACAG
CCGGAGTTTACCTCACTCTCCAATTAACGGATTTTCTTTCTGAGTTAGTCACATCTTCTC
TATAGCTAAATCTGAAAGTTTTACATATGTTTATGAAAATTATGAATTCATATTGAAAGC
TCTCATACTCTACACAGACTGCACTCTTAGTTAAATTCAGCTCAAAAGATTTCTACATTG
TAAGCGTCTCTGTAATTTCTCCTGTACAGTAGCACTATCTTCTATTACATATTACTCTCC
CAGTGTCTTCCTTTTGAGTGGTCCTGTATTACATTAGCAGGTTCTTCTTGCTTTGACTTC
ACCTTCTTACCACGGACCGGTAAGGGTTCTTCTCAAGCATTGTCTTTTGGCCAGCAATGG
AGGCTGCTTCGAAAATTCCTTTAGTGCCAAATTC

Last Updated on 06.12.2018 16:11