Sequences from confirmation amplicon


Confirmation sequences for line 552G05 and hit At5g03160 are shown.
Line ID 552G05
Locus At5g03160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:750192
Primers used for PCR MJ38 - 8474, show primer details
   
Sequence Name 37-K029914-022-552-G05C-8409
GenBank Accession LN502497 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K029914-022-552-G05C-8409
GCCATATTGAAGAAGAGTCTTTGATTCAGCAAATTTGTGTGTTTCGAATTATCGTTCTTC
GATCGAATTCGAAGCCTTTTTTTTATTTTGATTTGCATGTTTTAAGTGTAACTAGTTATA
ACAACACGTTGTTCTATCCAATCGTTATGACCCACGTGGAACTTGTTTTAAGTAAATTTC
AATTTCGTAAATAGTAACCAATGATAATTAAACACGTATGACTAACTATTCCACGCTGGC
TCTTACTCTGCCACGTAAGATAACTGCGATTCTTGGGCCTATTAGAATTGAAGCCTTAAA
GGCCAACCTTATAACTGAAAGATAAATATGGGCCTAACAGAATACTTGATGGGCCCAAAA
TGTTGGAAGTGTAATTAACCGGATCAGTATCACAAAACTTGCATCTAATTTTTTTTTTGC
TTCAGAT
   
Sequence Name 37-K029914-022-552-G05C-MJ38
GenBank Accession LN502498 [GenBank]
Sequencing primer gene specific primer, MJ38: 5'-ATTCTTTCCAAAATCTGAAGCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K029914-022-552-G05C-MJ38
GATCCGGTTAATTACACTTCCACATTTTGGGCCCATCAAGTATTCTGTTAGGCCCATATT
TATCTTTCAGTTATAAGGTTGGCCTTTAAGGCTTCAATTCTAATAGGCCCAAGAATCGCA
GTTATCTTACGTGGCAGAGTAAGAGCCAGCGTGGAATAGTTAGTCATACGTGTTTAATTA
TCATTGGTTACTATTTACGAAATTGAAATTTACTTAAAACAAGTTCCACGTGGGTCATAA
CGATTGGATAGAACAACGTGTTGTTATAACTAGTTACACTTAAAACATGCAAATCAAAAT
AAAAAAAAGGCTTCGAATTCGATCGAAGAACGATAATTCGAAACACACAAATTTGCTTGA
ATCAAAGACTCTTCTTCAATATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTA
CGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTA
TTTCGACGTGTCTACATTC
Primers used for PCR MJ39 - 8474, show primer details
   
Sequence Name 60-K029918-022-552-G05C-8409
GenBank Accession LN510156 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K029918-022-552-G05C-8409
TCGTTCGTTCGTTTCGTGATTTCCAAGAATTCCAAATTTGTCGGTGTAATCTTCGTTGTA
TTCAGAGGTGTATGTATATGTATTGAAATCGAGAATGGATCTGTTCCGAGTTTGGTCAGG
GATGGATTTCTTAGCTTGGAGAGGAATGGCTTACACACTTCTTCTTCTCAACTTCGTCTT
TGCTTGCCAACTTCTTCTTCTCCAACCTCTCGTTTCCGCTCTAGGTATATTTTATTCTCT
TTGATTTTCCTATCTTCGCTTCGTTTTCCCCTGGTGAGATTGATGTTTGTTGTTTAGCTG
ATTTGGGAATCTTAGGGCTTAGAAATTTGCTCTAAATGGCGAATTGCTTCTGTACTTTTC
GAAAGTTTTCTCCTTTTCGTGTTGGGCTTGTCGCAAATTGAGGTTCCCTGTGATGGTATT
GAAATCGAATTTAGGACTCTGTTTTTGCTGCTCCACATTATGAATTGATCTAATTTGGAT
ACTAGGTTATGAGAGTAATGCATCTTTGTATACTCGTTGAGTGTTAGCGTTGCTGTTTAT
TTTGGTACAGATGGTCAATCGGTA
   
Sequence Name 60-K029918-022-552-G05C-MJ39
GenBank Accession LN510157 [GenBank]
Sequencing primer gene specific primer, MJ39: 5'-AATAACTCAGCAGCATCTACCGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K029918-022-552-G05C-MJ39
TACAGCACGCTACACTCAACGAGTATACAAAGATGCATTACTCTCATAACCTAGTATCCA
AATTAGATCAATTCATAATGTGGAGCAGCAAAAACAGAGTCCTAAATTCGATTTCAATAC
CATCACAGGGAACCTCAATTTGCGACAAGCCCAACACGAAAAGGAGAAAACTTTCGAAAA
GTACAGAAGCAATTCGCCATTTAGAGCAAATTTCTAAGCCCTAAGATTCCCAAATCAGCT
AAACAACAAACATCAATCTCACCAGGGGAAAACGAAGCGAAGATAGGAAAATCAAAGAGA
ATAAAATATACCTAGAGCGGAAACGAGAGGTTGGAGAAGAAGAAGTTGGCAAGCAAAGAC
GAAGTTGAGAAGAAGAAGTGTGTAAGCCATTCCTCTCCAAGCTAAGAAATCCATCCCTGA
CCAAACTCGGAACAGATCCATTCTCGATTTCAATACATATACATACACCTCTGAATACAA
CGAAGATTACACCGACAAATTTGGAATCTTTGGAATCACTGAAACGAACGAACGAAGAAG
AAGAGAAAGAGTATAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGA
TCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTT
CGACGTGTCTAC

Last Updated on 06.12.2018 16:11