Sequences from confirmation amplicon


Confirmation sequences for line 556A06 and hit At5g62070 are shown.
Line ID 556A06
Locus At5g62070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24932161
Primers used for PCR 26ew - 8474, show primer details
   
Sequence Name 49-K110987-0022-556-A06C-26ew
GenBank Accession LN506877 [GenBank]
Sequencing primer gene specific primer, 26ew: 5'-TAAATGGTTTGAACAAAACCACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K110987-0022-556-A06C-26ew
TTGACTTTACCGACGCCCTTTAGATAAGTCAAGCTAAGCAAAAAATAAAAAAGCCTTTTA
AAATCTCGTGTAACTAACTCGCTGTATGCCGGCGCGTGGAAACGTACTCTGCAATCTATA
TATAAGCCGTTAAACTCTATTTAGCTTCTCTATAGACAAAAGTTTATGTTTTGTTTTTCC
TCTAACACAAAAAACAATCTCAGATATTAAATAAAGTGTTAATCAACCGGAGAACTCTCC
GACAAAACCTCGCCGACGATTTTCCGACGATGAAAGATGGGCTTTTTCGGGAGACTGTTC
GGGAGTAAAAAAAAGTCTGATAAAGCTGCTTCGTCGAGAGATAAACGGAGGTGGAGCTTC
ACCACCAGATCTTCAAATTCCAGCAAGAGAGCTCCGGCGGTGACGTCGGCTTCGGTGGTT
GAGCAAAATGGTTTGGATGCTGACAAACATGCGATAGCTGTGGCTGCTGCGACAGCCGCG
GTGGCGGAAGCAGCTCTTACTGCTGCTCATGCGGCAGCTGAAGTCGTGAGACTAACAAGC
GGAAACGGCGGGAGAAACGTCGGTGGCAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
   
Sequence Name 33-K110986-0022-556-A06C-8409
GenBank Accession LN501040 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K110986-0022-556-A06C-8409
CATTTACAATTGAATATATCCTGCCACCGACGTTTCTCCCGCCGTTTCCGCTTGTTAGTC
TCACGACTTCAGCTGCCGCATGAGCAGCAGTAAGAGCTGCTTCCGCCACCGCGGCTGTCG
CAGCAGCCACAGCTATCGCATGTTTGTCAGCATCCAAACCATTTTGCTCAACCACCGAAG
CCGACGTCACCGCCGGAGCTCTCTTGCTGGAATTTGAAGATCTGGTGGTGAAGCTCCACC
TCCGTTTATCTCTCGACGAAGCAGCTTTATCAGACTTTTTTTTACTCCCGAACAGTCTCC
CGAAAAAGCCCATCTTTCATCGTCGGAAAATCGTCGGCGAGGTTTTGTCGGAGAGTTCTC
CGGTTGATTAACACTTTATTTAATATCTGAGATTGTTTTTTGTGTTAGAGGAAAAACAAA
ACATAAACTTTTGTCTATAGAGAAGCTAAATAGAGTTTAACGGCTTATATATAGATTGCA
GAGTACGTTTCCACGCGCCGGCATACAGCGAGTTAGTTACACGAGATTTTAAAAGGCTTT
TTTATTTTTTGCTTAGCTTGACTTTATCTAAAGGGCGTCGGTTAAAGTCAATGGGCCTGA
CTAATCCTACCAACCGTACCAGATCTGTGCTTTGTGGTTTTGTTCAAACCATTT

Last Updated on 06.12.2018 16:11