Sequences from confirmation amplicon


Confirmation sequences for line 569D10 and hit At1g29910 are shown.
Line ID 569D10
Locus At1g29910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10472859
Primers used for PCR 33tq - 8474, show primer details
   
Sequence Name 85-K116920-0022-569-D10C-33tq
GenBank Accession LN514584 [GenBank]
Sequencing primer gene specific primer, 33tq: 5'-AGCTAGTGGACTAGAGACTGCCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K116920-0022-569-D10C-33tq
AATAACAAACGTTACTCGCAATTTTCCTATATAATCCAACCCTACCTAACCATTTTCAAT
CACTCTCACTCACAAGTTAGTCACCAAAAAAAAAAAAAACCCAAAAGTTTTCAGGGGGCC
CCCCCCAAGGGGGTCTCCCCCCCCCGGCCTTCCCGGGAAGGGGGGGAAACCTTTCCCCCG
GGGAATTAAAAATCCCCGAAAGGGGCCGGGGAAAAAAGGAAAAAATGGGTCCCAACCCAA
GGGGCCCCCCGGGGCCCCCGGGGGAGGGACCCCACCGGGTAAAGTTTGGGGGCCCTTTTC
GGGGGGGGTCCCCGGGCCCCCTTCCGGGAAATTCCCCCGGAAACCCGGGGGGGGACCCCC
GGGGATTTTCCGGGGACCCCAAAAATTTCCAAGGGAACCGGGGGCTAAAATTTTCCCCCC
GGGGGGGGGCTTTGCCGGGACCCCAGGGGGGGCTCCCCCCGGAATTTTGGGCAAAAAAGG
GATTCAATTTCGAAAGGGGGGTTGGGTTCAGGGGCGGTTCCAAAATTTTTGGGGGGGGGG
GACCCTTTTTCTGGGGACCTCCCCGGGGAACCCCCAGGGGTTGGGGGGCCAATTGGGAAA
AAAAAAAAAAATTTTCCCCTTTTTTTTTCCCATTCAAAAAGGGAAATCCCCCGCGCTAAA
AAAAAAAA
   
Sequence Name 41-K116919-0022-569-D10C-8409
GenBank Accession LN504226 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K116919-0022-569-D10C-8409
CCGCCTCTCCGAACTTGACTCCGTTCCTAGCCAAAAGCTCAGGGAAGACGCAGCCTAGGG
CTCCGAGCATAGCCCACCCGCTGTGGATAACTTCTAGCTCACGGTTCCTTGCGAATGTCT
CGGGATCAGCTGAAAGTCCAGCGGTGTCCCATCCGTAGTCTCCGGGGAACTCTCCGGTAA
GGTAGCTCGGTGACTCGCCAGAGAATGGACCCAAGTACTTGACTCGGTCGGATCCGTACC
ATGGGCTGCCTGATGGACCCTTTGGCTTGGCAACAGTCTTCCTCATTGTCACACGGCCGC
TTCCGAGGACTTCAGATGCCGCGGGGGAAAGGTTCACAGCCTTTCCGGCGAAGGCAGGGG
AGGAGAGAGCCATTGTGGAGGCGGCCATTGAAACTTTTTGTGTTTTTTTTTTTTTTGGGG
CCCAATTTGGGGGTGAAAATTTTTAAAAATGGTTTGGGGGGGTTGGTTTTTTAAGAAAAA
TTTGGGAATAACTTTTTTTTTTTTAAAAAAAACCTTTTTCCTTTTCCCCCGGGGATTTTT
AAAATTTTGGTTTTAACCTTTCCCTTTCCTAGGGGGG

Last Updated on 06.12.2018 16:11