Sequences from confirmation amplicon


Confirmation sequences for line 575C05 and hit At5g19070 are shown.
Line ID 575C05
Locus At5g19070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6376552
Primers used for PCR 52by - 8474, show primer details
   
Sequence Name 30-K108223-0022-575-C05C-52by
GenBank Accession LN499751 [GenBank]
Sequencing primer gene specific primer, 52by: 5'-CAATGAGCTACACTAACGGTGGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K108223-0022-575-C05C-52by
TATGTTATCCTAGATATAGGAACATGAGATTACCTTGGTACTGAACTTCTCTTTTTTTTT
TGCAATGTCTGCAAAGCTGTTGCGTACATTCCTCTAACAGTTTTGGCTGTTCCTGCCTCG
GTTCTCACGGTATATATACATATTTCTTTTGTGTGTGATATATTTACCGCTATCATCCAC
TCTCAACTTTCTTTGTTAAAGTGACTGGTTTATCTATAAAATTGAAAAATCTTATTTGGT
TGAAGCTGTGATATATTCAGCTCGGTGGTGGCTACCTTTTTGGGCTGCCAATTGGTTTTG
TGGCGGACTCAGTCGGTGCTACACTTGGCTCAGGAGCTGCATTTCTACTAGGCCGTACGG
TAAGCCACTCAAATTCTTATTGATTTGCAAACACAACCATTAAATTAATGAAGTGACATG
CATAAACCACTGACGCTATCACATGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAAATGTCCGCAGCGTTATT
   
Sequence Name 14-K108222-0022-575-C05C-8409
GenBank Accession LN492541 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K108222-0022-575-C05C-8409
GCCTTTACAATTGAATATATCCATGTGATAGCGTCAGTGGTTTATGCATGTCACTTCATT
AATCTAATGGTTGTGTCTGCAAATCAATAAGAATTTGAGTGGCTTACCGTACGGCCTAGT
AGAAATGCAGCTCCTGAGCCAAGTGTAGCACCGACTGAGTCCGCCACAAAACCAATTGGC
AGCCCAAAAAGGTAGCCACCACCGAGCTGAATATATCACAGCTTCAACCAAATAAGATTT
CTCAATTTTATAGATAAACCAGTCACTCTAACAAAGAAAGTTGAGAGTGGATGATAGCGG
TAAATATATCACACACAAAAGAAATATGTATATATACCGTGAGAACCGAGGCAGGAACAG
CCAAAACTGTTAGAGGAATGTACGCAACAGCTCTGCAGACATCGCAAAAAAAAAAGAGAA
GTTCAGTACCAAGGTAATCTCATGTTCCTATATCTATGGATTAACATTAGCAATGAAACA
GTGATTCAGCTACCACCGTTAGTGTAGCTCA

Last Updated on 06.12.2018 16:11