Sequences from confirmation amplicon


Confirmation sequences for line 602A05 and hit At4g13730 are shown.
Line ID 602A05
Locus At4g13730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7971041
Primers used for PCR 05kx - 8474, show primer details
   
Sequence Name 50-K113756-0022-602-A05C-05kx
GenBank Accession LN507236 [GenBank]
Sequencing primer gene specific primer, 05kx: 5'-ATAACACCCTTAGCTACTGGCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K113756-0022-602-A05C-05kx
CTTCATTGCTCTTTCTCTCCGGTTACAGAAATGCGACTGTGTCTAATCGACTCATTCTAG
CTAGCTATTACTTTACAAGAATGGCATCCACTCTAATCAGAAATTTGATGGAATATCCCG
AAGGGGTGATCAAAAGAGACTGCCGATTAAGCCAATTGTGTAACTCTCTTCACAAACCAA
TCAATACAATGTTGGAGTATCAAATCACCAAAACGGACTTACAGGGTTCATCAGAAGCTC
CTCTTTGAATTGCTTGTACTGAGACCTTTTCTTAGCTAACTCAGAAGACCACAGAGAGCG
ATCCGGTGACAAATAATCCAACAAAAGCTGTCAAATTCAAGAAAACAGCATGAAGCATAA
ACTGACAGAATTAGTAATCGGAGAAGAAACTGTATCGGAGAAGAAATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 12-K113755-0022-602-A05C-8409
GenBank Accession LN491659 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K113755-0022-602-A05C-8409
ATTTACATTGAATTTCTTCTCCGATACAGTTTCTTCTCCGATTACTAATTCTGTCAGTTT
ATGCTTCATGCTGTTTTCTTGAATTTGACAGCTTTTGTTGGATTATTTGTCACCGGATCG
CTCTCTGTGGTCTTCTGAGTTAGCTAAGAAAAGGTCTCAGTACAAGCAATTCAAAGAGGA
GCTTCTGATGAACCCTGTAAGTCCGTTTTGGTGATTTGATACTCCAACATTGTATTGATT
GGTTTGTGAAGAGAGTTACACAATTGGCTTAATCGGCAGTCTCTTTTGATCACCCCTTCG
GGATATTCCATCAAATTTCTGATTAGAGTGGATGCCATTCTTGTAAAGTAATAGCTAGCT
AGATTGATCGATTAGACACAGTCGCATTTCTGTTAACCGGAGAGAAATGAGCAATGAAGT
TAACATAGTGGCATACTGGCATGTCTGTTGCCAGTAGCTAGGGGGTGTTATA

Last Updated on 06.12.2018 16:11