Sequences from confirmation amplicon


Confirmation sequences for line 611E12 and hit At2g42350 are shown.
Line ID 611E12
Locus At2g42350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17639735
Primers used for PCR CH72 - 8474, show primer details
   
Sequence Name 03-K105749w-0022-611-E12C-CH72
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, CH72: 5'-TAACAACAAAACTGATGATTCCGA-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K105749w-0022-611-E12C-CH72
CGACTTCTTCGATGCCGCAGCAAGATTTTCCTCGTCACCACCGTTTCCTTCTCTATTATT
ATCATCATCGTGTTCGTTTATTATCTCTACGCAAAATTCGTTCTCCACCGTAGATCAGCC
TTCCAAGACCTCTCCTTCTCTGTCGTTTCTCAGCCGCCAAAACGTGGCCTCGATTCTCTA
GTCATCGCTTCTCTCCCAACGTTCGTGGTTGGAATCAAGAATGACGTGGCGGGTACGGAA
TGTGCGGTTTGTCTAAGCTTATTGGAGGAGAAAGATAATGCAAGGATGTTACCGAACTGC
AAACACGTGTTCCACGTGTCTTGCGTTGACACGTGGCTCACCACGCAGTCCACTTGTCCC
GTTTGTCGAACTGAAGCCGAGCCAAGTCATCCAAGGCTTGAGCCTGAGCCTAGGGAAGGG
CCAGTTGGCGACTTTGAAAACGTCACCAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 59-K105750-0022-611-E12C-8409
GenBank Accession LN510004 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K105750-0022-611-E12C-8409
GCCATTTACATTGAATATATCCTGGTGACGTTTTCAAGTCGCCAACTGGCCCTTCCCTAG
GCTCAGGCTCAAGCCTTGGATGACTTGGCTCGGCTTCAGTTCGACAAACGGGACAAGTGG
ACTGCGTGGTGAGCCACGTGTCAACGCAAGACACGTGGAACACGTGTTTGCAGTTCGGTA
ACATCCTTGCATTATCTTTCTCCTCCAATAAGCTTAGACAAACCGCACATTCCGTACCCG
CCACGTCATTCTTGATTCCAACCACGAACGTTGGGAGAGAAGCGATGACTAGAGAATCGA
GGCCACGTTTTGGCGGCTGAGAAACGACAGAGAAGGAGAGGTCTTGGAAGGCTGATCTAC
GGTGGAGAACGAATTTTGCGTAGAGATAATAAACGAACACGATGATGATAATAATAGAGA
AGGAAACGGTGGTGACGAGGAAAATCTTGCTGCTGGCATCGTAAGAAGTCGAGTTTTGCC
AGAAAGAACGGTCGTCGGAATCATCAGTTTTGGTTGTTA

Last Updated on 06.12.2018 16:11