Sequences from confirmation amplicon


Confirmation sequences for line 649H03 and hit At5g11730 are shown.
Line ID 649H03
Locus At5g11730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3782149
Primers used for PCR CQ51 - 8474, show primer details
   
Sequence Name 35-K107033-0022-649-H03C-8409
GenBank Accession LN501851 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K107033-0022-649-H03C-8409
TCGGACTGAGCCTTTACAATTGAATTATCCCGAAATTCAAGAGTTCTGCAGGCCTGCTTG
CTATGTGGACGAGCATTACTTCCCAACAATGTTGACGATTGAGAAACCAACAGTCTTGGC
TAACCGGAGCTTGACATGGGTGGACTGGTCGAGGGGCGGTCCCCACCCAGCTACGTTTGG
GCGATCGGACATTACAGAAAACTTCTTTGGAAAAATCTTTGATGGAAGAAACTGCAGTTA
CAATGGTCGCAACACTTCCATGTGTTATCTCTTTGCGAGGAAGTTTGCTCCGAGTGCTTT
GGAGCCTTTGCTTCACATTGCTCCCAAGATTCTTGGATTTTGAGTAGTTTAGTTATGTCT
CTGAAGTCTGAACCTCTTGACATCAAATATTTTATTTTGTTTGTTGTGATACTAGTTTGG
AAGAAATGAGATTTTAGTAGAGAGAACTAGGTTGCACGACAAACAAAATAAAATATTTGA
TGTCAAGAGGTTCAGACTTCAGAGACATAACTAAACTACTCAAAATCCAAGAATCTTGGG
AGCAATGTGAAGCAAAGGCTCCAAAGCACTCGGAGCAAACTTCCTCGCAAAGAGATAACA
CATGGAAGTGTTGCGACCATTGTAACTGCAGTTTCTTCCATCAAAGATTTTTCCAAAGAA
GTTT
   
Sequence Name 43-K107034-0022-649-H03C-CQ51
GenBank Accession LN504852 [GenBank]
Sequencing primer gene specific primer, CQ51: 5'-TGTCGTGCAACCTAGTTCTCTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K107034-0022-649-H03C-CQ51
AACAAACAAATAAAATATTTGATGTCAAGAGGTTCAGACTTCAGAGACATAACTAAACTA
CTCAAAATCCAAGAATCTTGGGAGCAATGTGAAGCAAAGGCTCCAAAGCACTCGGAGCAA
ACTTCCTCGCAAAGAGATAACACATGGAAGTGTTGCGACCATTGTAACTGCAGTTTCTTC
CATCAAAGATTTTTCCAAAGAAGTTTTCTGTAATGTCCGATCGCCCAAACGTAGCTGGGT
GGGGACCGCCCCTCGACCAGTCCACCCATGTCAAGCTCCGGTTAGCCAAGACTGTTGGTT
TCTCAATCGTCAACATTGTTGGGAAGTAATGCTCGTCCACATAGCAAGCAGGCCTGCAGA
ACTCTTTGAATTTCGGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT

Last Updated on 06.12.2018 16:11