Sequences from confirmation amplicon


Confirmation sequences for line 673E01 and hit At1g34245 are shown.
Line ID 673E01
Locus At1g34245 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12473596
Primers used for PCR BF40 - 8474, show primer details
   
Sequence Name 27-K029004-022-673-E01C-8409
GenBank Accession LN498248 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K029004-022-673-E01C-8409
TTTTTGAATTATATATCCTGCGGACGGATTGTTCATTGCTGATCCATGTAGATTACAGGA
TCCATGTAGAGGGAGATACTATCACGTGCCATCTAGAGCTTGAATTGAATCTCCGCCGTT
TATTTTTTTTTGACGTCTGTGATTAAAATATCAGTTTTGGCTGGCGGCGAAACTTTTTTT
CTATTGTACAATTTGTTGGGATATTAAATTAAATGCTATTGGCCCACTTTGAAGTCTTAT
AATTAATATTGGGCTTTATTAGGCCTTTTCAAAGAGAGTTTGTATGACACGACCGAATCA
GATTCTTGAACCGGTACATCACAAAGTCAAATGCTTCGGTTGGCCAATTATTTATTGTTT
CCTTTTAAAAGCCATCTCCATCATACCGGTAACCGGTTAAACCAAAAAAATCATTTATGT
AACAATGTGTAAAGTATAAACTTTTTCTTACTATGGATCGTTTTGAA
   
Sequence Name 28-K029004-022-673-E01C-BF40
GenBank Accession LN498711 [GenBank]
Sequencing primer gene specific primer, BF40: 5'-AAGATGTAAATTTCAAAACGATCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K029004-022-673-E01C-BF40
CACATTGTTACTAAATGATTTTTTTGGTTTAACCGGTTACCGGTATGATCGAGATGGCTT
TTAAAAGGAAACAATAAATAATTGGCCAACCGAAGCATTTGACTTTGTGATGTACCGGTT
CAAGAATCTGATTCGGTCGTGTCATACAAACTCTCTTTGAAAAGGCCTAATAAAGCCCAA
TATTAATTATAAGACTTCAAAGTGGGCCAATAGCATTTAATTTAATATCCCAACAAATTG
TACAATAGAAAAAAAGTTTCGCCGCCAGCCAAAACTGATATTTTAATCACAGACGTCAAA
AAAAAATAAACGGCGGAGATTCAATTCAAGCTCTAGATGGCACGTGATAGTATCTCCCTC
TACATGGATCCTGTAATCTACATGGATCAGCAATGAACAATCCGTCCGCAGGATATATAA
ATTCAAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTA
TTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTC
TACATTC

Last Updated on 06.12.2018 16:11