Sequences from confirmation amplicon


Confirmation sequences for line 684B07 and hit At2g43750 are shown.
Line ID 684B07
Locus At2g43750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18131773
Primers used for PCR GC50 - 8474, show primer details
   
Sequence Name 19-K033540-022-684-B07C-8409
GenBank Accession LN494660 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K033540-022-684-B07C-8409
ATTTACATTGAATATATCCTGATACACAAACCTATCCTTGACACTGCAACATGGTTCCAT
GATCTCAAGCTTAGCAGCAACACTTGCAACACAGCCCTTGACTACATTGTTCAAGTACAC
CATCGGAGTTTTCCCAATAAGCTGAATCAATTCATAACATTCAGAAAGAATATAAAAAAA
TTAGAAACGAAATCAAAATTCAAAGAAGTCAAATTTACAAGACTAATAATACTCAGTTAG
TTTATATCTTCCCCTAAATTGACAAAATTGATCACCAATTTAGATTTCCTCTGTCTCTAT
CCAAATGCAAACGAAACACAATCTAACCTGAGCGGCGTTATCGGCGATATTGAGCCCTTC
AACACCAGCTTCTGGCTTGATAGATACAGCCTTGCACACAACATCACTCCGGCTCTGTCT
CTTGAGCGTGAAAGCAGCTGAGGAAACATCGAAAGCAGCAGCCTTTCGAGAGGATAAGGA
GAGAGAAGAGAGCTCTGGCGAGTATTTGAAAGGACGGTGGCGAGAAGTCAACGGATTAAG
GAGAAAAGCAGAGGAAGATGTCGCCGCCATGACTTGCTCTTTTGCTTCAGTGTATCTT
   
Sequence Name 19-K033540-022-684-B07C-GC50
GenBank Accession LN494661 [GenBank]
Sequencing primer gene specific primer, GC50: 5'-TTCGGAAAGTGAGGAAGATACACT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K033540-022-684-B07C-GC50
CGGCGACTCTTCCTCTGCTTTTCTCCTTAATCCGTTGACTTCTCGCCACCGTCCTTTCAA
ATACTCGCCAGAGCTCTCTTCTCTCTCCTTATCCTCTCGAAAGGCTGCTGCTTTCGATGT
TTCCTCAGCTGCTTTCACGCTCAAGAGACAGAGCCGGAGTGATGTTGTGTGCAAGGCTGT
ATCTATCAAGCCAGAAGCTGGTGTTGAAGGGCTCAATATCGCCGATAACGCCGCTCAGGT
TAGATTGTGTTTCGTTTGCATTTGGATAGAGACAGAGGAAATCTAAATTGGTGATCAATT
TTGTCAATTTAGGGGAAGATATAAACTAACTGAGTATTATTAGTCTTGTAAATTTGACTT
CTTTGAATTTTGATTTCGTTTCTAATTTTTTTATATTCTTTCTGAATGTTATGAATTGAT
TCAGCTTATTGGGAAAACTCCGATGGTGTACTTGAACAATGTAGTCAAGGGCTGTGTTGC
AAGTGTTGCTGCTAAGCTTGAGATCATGGAACCATGTTGCAGTGTCAAGGATAGGTTTGT
GTATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAA
ATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATC
TTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11