Sequences from confirmation amplicon


Confirmation sequences for line 692D12 and hit At1g48800 are shown.
Line ID 692D12
Locus At1g48800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18051268
Primers used for PCR 26pk - 8474, show primer details
   
Sequence Name 62-K115187-0022-692-D12C-26pk
GenBank Accession LN510822 [GenBank]
Sequencing primer gene specific primer, 26pk: 5'-ACTAGGTTTGAGGGGAAAACAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K115187-0022-692-D12C-26pk
AATAACGCATAAAAACAAAGTTGTAACTCCTAAACTTTTTTCATTGTTCAATATAGAAAA
TAATACAAGGTTACTATTTACCTTCTTAGGAAAAAAAAAACGTTTAAAAGAATATTTGGA
TATGATGTAAAAAAACATGAGAGAGTCCTGCTTGTGAGCATGATCGATGTTCGTACACAA
GTCTTTCGTACACAAGTCTTTTTTTTTAATTTTGACAGTCTTATAAGTTATAACTAGATT
CGTATATTTAACAAAAGGACACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAAATTTC
   
Sequence Name 26-K115186-0022-692-D12C-8409
GenBank Accession LN498055 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K115186-0022-692-D12C-8409
TCCTGTGTCCTTTTGTTAATATACGAATCTAGTTATAACTTATAAGACTGTCAAAATTAA
AAAAAAAGACTTGTGTACGAAAGACTTGTGTACGAACATCGATCATGCTCACAAGCAGGA
CTCTCTCATGTTTTTTTACATCATATCCAAATATTCTTTTAAACGTTTTTTTTTTCCTAA
AAAGGTAAATAGTAACCTTGTATTATTTTCTATATGGAACAATGAAAAAAGTTTAGGAGT
TACAACTTTGTTTTTTATGCGTTAATTTCATAATAAATAAATTTGAGGACCTCATATAAT
TTGTTTTCC

Last Updated on 06.12.2018 16:11