Sequences from confirmation amplicon


Confirmation sequences for line 693A09 and hit At1g01040 are shown.
Line ID 693A09
Locus At1g01040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28299
Primers used for PCR HM28 - 8474, show primer details
   
Sequence Name 23-K032600-022-693-A09C-8409
GenBank Accession LN496451 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K032600-022-693-A09C-8409
TTGAAATATCCTGCCATCATACTCATTTACTCTTTCATTTGCTGTCACTATTTTCCCAAT
AAGATCTTCTGCAACCATGCACCCATCAGCCATCATCAATTTTCCTTTTGATAAATCCTC
TTCTACTTCCTTCTCAAAAGCATCTCTCACAGGTAACAATCCAGAAGCTCTCACAACATC
GAAGGATGCAACAGCTCCTCTAATACCATAAGTTGGGTGAGATTTCTGCCCAAATACAAT
GTTGTGACGAAGTTTACCAAACCCATATTCCCTTCTGTCCCCACCAAGAGTTCTCTCATT
AGTCCCGAGATATACATCGGGACGAGCTCTCTGAAGAGGGTTGTCCCACGCTGTGGTTTT
CGTAATCTTTTCAACCAATTCCCAGTTGATCCCTTTTATGGGTTCCATAGA
   
Sequence Name 23-K032600-022-693-A09C-HM28
GenBank Accession LN496452 [GenBank]
Sequencing primer gene specific primer, HM28: 5'-ACTGACAATACGTCTATGGAACCC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K032600-022-693-A09C-HM28
GGTTGAAGATACGAAAACCACAGCGTGGGACAACCCTCTTCAGAGAGCTCGTCCCGATGT
ATATCTCGGGACTAATGAGAGAACTCTTGGTGGGGACAGAAGGGAATATGGGTTTGGTAA
ACTTCGTCACAACATTGTATTTGGGCAGAAATCTCACCCAACTTATGGTATTAGAGGAGC
TGTTGCATCCTTCGATGTTGTGAGAGCTTCTGGATTGTTACCTGTGAGAGATGCTTTTGA
GAAGGAAGTAGAAGAGGATTTATCAAAAGGAAAATTGATGATGGCTGATGGGTGCATGGT
TGCAGAAGATCTTATTGGGAAAATAGTGACAGCAAATGAAAGAGTAAATGAGTATGATGG
CAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTA
CGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTA
TTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11