Sequences from confirmation amplicon


Confirmation sequences for line 708F03 and hit At1g44080 are shown.
Line ID 708F03
Locus At1g44080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:16754312
Primers used for PCR CR36 - 8474, show primer details
   
Sequence Name 27-K107199-0022-708-F03C-CR36
GenBank Accession LN498412 [GenBank]
Sequencing primer gene specific primer, CR36: 5'-CATCTGGAAACAACTTTGATGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K107199-0022-708-F03C-CR36
AAATATTTGCTGGTATATGCTACAATCATCAAGCTTAACACACTAACATCTTCCCGGTCG
TTGAAGTAAATAGCATTCTCCAAACATCCATAGTACTCGGAGGCTAAGACCAAAAAACAA
TTGTCCGTCGCCACAATGAGTGCCTTGTCTCCCAAGGAACTGACCTCAACCCATTCTACC
AAATTCTTGTCCATCTTATAAACCTTAAAACAAACGGTTCTCTCGGTAAGAACTCGCTTT
ACGCAAAATTTCTTATAGAATCGATGAACAACGCATAGTTCTCCACAATACTCCACGAGT
CTCTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCACANTTATT
   
Sequence Name 11-K107198-0022-708-F03C-8409
GenBank Accession LN491138 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K107198-0022-708-F03C-8409
TTGAATATATCCTGAAGAGACTCGTGGAGTATTGTGGAGAACTATGCGTTGTTCATCGAT
TCTATAAGAAATTTTGCGTAAAGCGAGTTCTTACCGAGAGAACCGTTTGTTTTAAGGTTT
ATAAGATGGACAAGAATTTGGTAGAATGGGTTGAGGTCAGTTCCTTGGGAGACAAGGCAC
TCATTGTGGCGACGGACAATTGTTTTTTGGTCTTAGCCTCCGAGTACTATGGATGTTTGG
AGAATGCTATTTACTTCAACGACCGGGAAGATGTTAGTGTGTTTAAGCTTGATGATTGTA
GCATTATTACCAAGCAAATTAATTCTTCTTCTTCATCTTCTCATCAAAGTTGTTTCCAGA
TG

Last Updated on 06.12.2018 16:11