Sequences from confirmation amplicon


Confirmation sequences for line 710E12 and hit At3g61113 are shown.
Line ID 710E12
Locus At3g61113 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22615146
Primers used for PCR 28us - 8474, show primer details
   
Sequence Name 53-K117387-0022-710-E12C-28us
GenBank Accession LN508278 [GenBank]
Sequencing primer gene specific primer, 28us: 5'-GTATCGAGCTGACCACTAAGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K117387-0022-710-E12C-28us
GCCTCCTGCGTTTATGAATTCCAACCACCATTGTTATTAAACACTAAAAACAGAGTTCTT
GCTGGAAATCAAGGAAAGAAAAGAGAGAAGCTTTACACGGTATCGCCTTTCATGAACATT
TCAGGCCTTTCCTTGATCAAATTGGTACGAACCCATGAAAGCAAATCCTTCATTGTCAAC
TGTACTGAATGAAACACCAGTGAGAAGTTTTACATGACTATAATAAACTCAATGGTATTT
AAAAATCATCAAAAGAGAGAAGCTTACGATATCAGAATCATTGAGTAAGTTGATGTTAAC
TTTATGAATCTTTACGGAGTCACAGAGCAATTCTAAACCTCCACTGCAGGAGATCAACAA
GCACATATTTTCATGTTAATACCAATACGAAACTCTCATTCACTACCAATTGATCTGATG
ATGACCGGAATGTAAAAAGTCTAAAGTCATTATAATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 26-K117386-0022-710-E12C-8409
GenBank Accession LN498106 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K117386-0022-710-E12C-8409
ACATTGAATATTATAATGACTTTAGACTTTTTACATTCCGGTCATCATCAGATCAATTGG
TAGTGAATGAGAGTTTCGTATTGGTATTAACATGAAAATATGTGCTTGTTGATCTCCTGC
AGTGGAGGTTTAGAATTGCTCTGTGACTCCGTAAAGATTCATAAAGTTAACATCAACTTA
CTCAATGATTCTGATATCGTAAGCTTCTCTCTTTTGATGATTTTTAAATACCATTGAGTT
TATTATAGTCATGTAAAACTTCTCACTGGTGTTTCATTCAGTACAGTTGACAATGAAGGA
TTTGCTTTCATGGGTTCGTACCAATTTGATCAAGGAAAGGCCTGAAATGTTCATGAAAGG
CGATACCGTGTAAAGCTTCTCTCTTTTCTTTCCTTGATTTCCAGCAAGAACTCTGTTTTT
AGTGTTTAATAACAATGGTGGTTTGGAATTCATAAACGCAGGAGGCCTGGAGTTCTCGTG
CTGGTTAATGACTGCGATTGGGAACTTAGTGG

Last Updated on 06.12.2018 16:11