Sequences from confirmation amplicon


Confirmation sequences for line 721A06 and hit At3g56100 are shown.
Line ID 721A06
Locus At3g56100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20817804
Primers used for PCR 14bc - 8474, show primer details
   
Sequence Name 62-K107060-0022-721-A06C-8409
GenBank Accession LN510769 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K107060-0022-721-A06C-8409
CGGAATGAGCCTTTACAATTGAATATCAATTGGCAAGGCTTCCTCTAGACATGTAATCGA
AGACGACAAGCTTCTCTCCTTTAGGGCCTAAGTAATAAGCTCTGAGTGCAAGGAGATTCG
GATGCCGGATTCTTCCCAAGACGTTAATCTCATTCTCAAACTCTTTTTGACTTTTGGTGA
TCTTTTCTCTCAATCTCTTCACTGCTACTTGACTTCCATCTTCAAGTGTAGCTTTGTACA
CAGTCCCATAAGTGCTTTTCCCCATTATCTCTGCTGTTGCACACAAAAGATCATCTGCAG
TAAACGCCATTGGTCCATCAAAATGAACCAGCTTCCCTCCAGTTTCACCTCCAGCTTCAG
CTTCCCCTCCTTTCTCAGTCTTTGCGGCTACAGCTCCAGGTCCAGCCTCTCCGCCCTTGG
CTTTGGTTTCATTGGCTTTCTTCCTCAGCAAGCAACATAGA
   
Sequence Name 54-K107061-0022-721-A06C-14bc
GenBank Accession LN508494 [GenBank]
Sequencing primer gene specific primer, 14bc: 5'-TCCTTGTTTGTGTTCTATGTTGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K107061-0022-721-A06C-14bc
ACAAGCCAAGGGCGGAGAGGCTGGACCTGGAGCTGTAGCCGCAAAGACTGAGAAAGGAGG
GGAAGCTGAAGCTGGAGGTGAAACTGGAGGGAAGCTGGTTCATTTTGATGGACCAATGGC
GTTTACTGCAGATGATCTTTTGTGTGCAACAGCAGAGATAATGGGGAAAAGCACTTATGG
GACTGTGTACAAAGCTACACTTGAAGATGGAAGTCAAGTAGCAGTGAAGAGATTGAGAGA
AAAGATCACCAAAAGTCAAAAAGAGTTTGAGAATGAGATTAACGTCTTGGGAAGAATCCG
GCATCCGAATCTCCTTGCACTCAGAGCTTATTACTTAGGCCCTAAAGGAGAGAAGCTTGT
CGTCTTCGATTACATGTCTAGAGGAAGCCTTGCCAATTGATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11