Sequences from confirmation amplicon


Confirmation sequences for line 725A02 and hit At1g01040 are shown.
Line ID 725A02
Locus At1g01040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28250
Primers used for PCR IF13 - 8474, show primer details
   
Sequence Name 62-K032734-022-725-A02C-IF13
GenBank Accession LN510674 [GenBank]
Sequencing primer gene specific primer, IF13: 5'-TGTCAGATGTGTAGATTTTGGCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K032734-022-725-A02C-IF13
TCGCGATTCTTTTTGGCAATGAGCTGGATGCAGAGGTTGTTGTTTCTCTCTCCATGGATT
TGAGTATTAGAATTTGAAGATGTAACCATGGTTAACTAAGATTTGTTTTACTGTATCAGG
TATTATCGATGTCTATGGATCTTTATGTTGCTCGGGCCATGATCACTAAAGCATCTCTTG
CTTTCAAGGGATCACTTGATATTACAGAAAACCAGGTTAGACTGAACAACAACCTCTATT
TTTCCTAACATTTATGTTTGCTTGAGAAACTTTTTATCTTATATTGGTGGCATTTCTTGT
GCAGCTATCATCTCTAAAAAAGTTTCATGTGAGGTTAATGAGTATCGTGTTGGATGTTGA
TGTTGAACCCTCCACGACACCATGGGATCCTGCAAAGGCCTACCTGTTTGTCCCTGTTAC
TGACAATACGTCTATGGAACCCATAAAAGGGATCAACTGGGAATTGGTTGAAAAGATTAC
GAAAACCACAGCGTGGGACAACCCTCTTCAGAGAGCTCGTCCCGATGTATATCTCGGGAC
TAATGAGAGAACTCTTGGTGGGGACAGAAGGGAATATGGGTTTGGTAAACTTCGTCACAA
CATTGTATTTGGGCAGAAATCTCACCCAACTTATGGTATTAGAGGAGCTGTTGCATCCTT
CGATGTTGTGAGAGCTTCTGGATTGTTACCTGTGAGAGATGCTTTTGAGAAGGAAGTAGA
AGAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
ATATGAGAAAAAGAAAGAGTAATTACCAATTTTTTTCAATTCAAAATGTAGATGTCCGCA
GCGTTATATAAAATGAAGTACATTT
   
Sequence Name 30-K032734-022-725-A02C-8409
GenBank Accession LN499613 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K032734-022-725-A02C-8409
CGGAATGAAGCATTTACTCTTCTACTTCCTTCTCAAAAGCATCTCTCACAGGTAACAATC
CAGAAGCTCTCACAACATCGAAGGATGCAACAGCTCCTCTAATACCATAAGTTGGGTGAG
ATTTCTGCCCAAATACAATGTTGTGACGAAGTTTACCAAACCCATATTCCCTTCTGTCCC
CACCAAGAGTTCTCTCATTAGTCCCGAGATATACATCGGGACGAGCTCTCTGAAGAGGGT
TGTCCCACGCTGTGGTTTTCGTAATCTTTTCAACCAATTCCCAGTTGATCCCTTTTATGG
GTTCCATAGACGTATTGTCAGTAACAGGGACAAACAGGTAGGCCTTTGCAGGATCCCATG
GTGTCGTGGAGGGTTCAACATCAACATCCAACACGATACTCATTAACCTCACATGAAACT
TTTTTAGAGATGATAGCTGCACAAGAAATGCCACCAATATAAGATAAAAAGTTTCTCAAG
CAAACATAAATGTTAGGAAAAATAGAGGTTGTTGTTCAGTCTAACCTGGTTTTCTGTAAT
ATCAAGTGATCCCTTGAAAGCAAGAGATGCTTTAGTGATCATGGCCCGAGCAACATAAAG
ATCCATAGACATCGATAATACCTGATACAGTAAAACAAATCTTAGTTAACCATGGTTACA
TCTTCAAATTCTAATACTCAAATCCATGGAGAGAGAAACAACAACCTCTGCATCCAGCTC
ATTGCCAAAAAGAATCGCGAACTCTGAAACTTCGCTTAGGAATGGATCTTTTGAAGAGCC
AA
Primers used for PCR AY38 - 8474, show primer details
   
Sequence Name 55-K118471-0022-725-A02C-AY38
GenBank Accession LN508941 [GenBank]
Sequencing primer gene specific primer, AY38: 5'-ACCTTTGATGTGGGAATAGGATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K118471-0022-725-A02C-AY38
CTCTCACTCTTCTCATTATAGAGGGTAACCTCTGAGCACCTCGGATAAGTGAACCCGAAA
GCGGATGCACAACGCATAGTTCAGGTGGAAGAAACACGTAATATGTCTTATCAAGGACTG
TCTCAGATTCACCTTTGAATTGCAAGAAAAAACAAAGAAGCGTTAATTTATCGGTTATAT
CATTAAATTGTTAAAGTGAAAAGAATTTCTTATAACCTGACTGTTCAAACCGAGGAGAAA
GAAGGTTCTTGCAATACGAAACACCACGTCCTTTAATCAAAGGTTGTTGCTTACAGTTCA
AATCAACTCCATACCTAATAAATAACATTATAGAAGAAGACTTGTATAGCCTCAATAACC
TCAGGTTGTGCAAGAAGTAGAAGAAAAGCCGGTCTTGTACAACATATATCCTAGCCATAG
AAGTAGCTATCTCATAATACATAGACAGACTTTGCTTCTCCTTCTTGCACAACACCACAG
GTGACTTGAGGCCATGTAAGTCCTCGTGATATTAGAAAAGTATTAGTATCTAATGAAACT
TAGAAAAAGGATTGAAGAAATCAAAAAATTATTTGAAGATGTTTTCTTACTTTTGCTTGT
AATAGTCAGCGTACGTGTTGTACTCTAGGGGACCAAGATATCCCTCTTTCCTAGGGAAAG
ATGTTTCTGCACTCATGTCATAACAAATTGAATCTACGTAAAACCGCTTCCCGGAATGTG
CGGCTGTCACTATNTTCCCAATAAGATCTTCTGCAACCATGCACCCATCAGCAATGAGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAATGTA
   
Sequence Name 23-K118470-0022-725-A02C-8409
GenBank Accession LN496759 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K118470-0022-725-A02C-8409
ATTTACTCATTGCTGATGGGTGCATGGTTGCAGAAGATCTTATTGGGAAAATAGTGACAG
CCGCACATTCCGGGAAGCGGTTTTACGTAGATTCAATTTGTTATGACATGAGTGCAGAAA
CATCTTTCCCTAGGAAAGAGGGATATCTTGGTCCCCTAGAGTACAACACGTACGCTGACT
ATTACAAGCAAAAGTAAGAAAACATCTTCAAATAATTTTTTGATTTCTTCAATCCTTTTT
CTAAGTTTCATTAGATACTAATACTTTTCTAATATCACGAGGACTTACATGGCCTCAAGT
CACCTGTGGTGTTGTGCAAGAAGGAGAAGCAAAGTCTGTCTATGTATTATGAGATAGCTA
CTTCTATGGCTAGGATATATGTTGTACAAGACCGGCTTTTCTTCTACTTCTTGCACAACC
TGAGGTTATTGAGGCTATACAAGTCTTCTTCTATAATGTTATTTATTAGGTATGGAGTTG
ATTTGAACTGTAAGCAACAACCTTTGATTAAAGGACGTGGTGTTTCGTATTGCAAGAACC
TTCTTTCTCCTCGGTTTGAACAGTCAGGTTATAAGAAATTCTTTTCACTTTAACAATTTA
ATGATATAACCGATAAATTAACGCTTCTTTGTTTTTTCTTGCAATTCAAAGGTGAATCTG
AGACAGTCCTTGATAAGACATATTACGTGTTTCTTCCACCTGAACTATGCGTTGTGCATC
CGCTTTCGGGTTCACTTATCCGAGGTGCTCAGAGGTTACCCTCTATAATGAGAAGAGTTG
AGAGCATGTTACTCGCTGTTCAAACTCAAAATTTGATTAGTTATCCTAT

Last Updated on 06.12.2018 16:11