Sequences from confirmation amplicon


Confirmation sequences for line 733A04 and hit At5g44870 are shown.
Line ID 733A04
Locus At5g44870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18116504
Primers used for PCR BE71 - 8474, show primer details
   
Sequence Name 23-K029489-022-733-A04C-8409
GenBank Accession LN496432 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K029489-022-733-A04C-8409
CCATTTACAATTGAATATATCTTGATAGTTCCACAGCCTGAGATGAACCCTTGTATCTTC
AGTCAATGCTTGTGAAGCAAGTTCCTTGGCGAATGTGCATAGTATATCATGCATCTCAAC
TCGACCACCAGAAATATTGACAAGAAACTTTCCTTTGAGATCTGTTATTTCATCCCAACT
CTTAGTAGACTCAGAATCACATGAATTCACCACGTGTCGTACATAACTTTCATTCTCTGA
TTTGAAGAAACATGCGACGTCAAGAAAAATATCTTTCTGCCTCTCTGTGAGTTCATCATA
CCTTCTTCTCAAGACATCTTGGATCATCTTATTGGAAATTAGTGTCAATGTTTTTATTCT
CTTTTCCCAATCAGCCTTGTCTTTCCCACAAAGTTCTACACCAAAAGCTCCGAGGGCTAG
TGGGTTGCCTTTGGCATAATTCAGAAAGTGTTTGGACAACTTTACCAAGTTTCCCTGGGC
ATCATCCAAACCAAATGCATGATTAGTAAACCACAGTAGACTGTCTCTGCTGTTCAAACT
TGGGACTACATAAGTATCTTTAACGAAACCCTTGAGCATTGACTCATCACTTGACGTAAT
AACAATCTTGCTTCCATTT
   
Sequence Name 24-K029489-022-733-A04C-BE71
GenBank Accession LN496875 [GenBank]
Sequencing primer gene specific primer, BE71: 5'-GAATTGGATTAAAAATGGAAGCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K029489-022-733-A04C-BE71
GTCAGTGATGAGTCATGCTCAGGGTTTCGTTAAAGATACTTATGTAGTCCCAAGTTTGAA
CAGCAGAGACAGTCTACTGTGGTTTACTAATCATGCATTTGGTTTGGATGATGCCCAGGG
AAACTTGGTAAAGTTGTCCAAACACTTTCTGAATTATGCCAAAGGCAACCCACTAGCCCT
CGGAGCTTTTGGTGTAGAACTTTGTGGGAAAGACAAGGCTGATTGGGAAAAGAGAATAAA
AACATTGACACTAATTTCCAATAAGATGATCCAAGATGTCTTGAGAAGAAGGTATGATGA
ACTCACAGAGAGGCAGAAAGATATTTTTCTTGACGTCGCATGTTTCTTCAAATCAGAGAA
TGAAAGTTATGTACGACACGTGGTGAATTCATGTGATTCTGAGTCTACTAAGAGTTGGGA
TGAAATAACAGATCTCAAAGGAAAGTTTCTTGTCAATATTTCTGGTGGTCGAGTTGAGAT
GCATGATATACTATGCACATTCGCCAAGGAACTTGCTTCACAAGCATTGACTGAAGATAC
AAGGGTTCATCTCAGGCTGTGGAACTATCAAGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGA
AAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAA
ACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTA

Last Updated on 06.12.2018 16:11